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- Objective:To construct an eukaryotic expressing vector pIRES1neo/hFL and express hFL in COS 7 cell. 目的 :构建人FL真核表达载体 pIRES1neo/hFL ,观察其在COS 7细胞中的表达。
- The insect expressing vectors could express Cyclin D1 and CDK4 proteins which possessed ability of phosphorylating Rb protein, showing biological activity. 研究表明 ;昆虫细胞表达的细胞周期蛋白D1和CDK4蛋白能促进Rb蛋白的磷酸化 ;具有生物活性 .
- The basic properties of the Pichia Pastoris, strains, express vectors, influencing factors on the express of foreign proteins and its aP.pastor... 本文就巴斯德酵母细胞的基本特性、宿主菌及其质粒、影响外源蛋白表达的因素和在医学上的应用作一综述。
- Finally a reshaping scFv 2F3 library were constructed by inserting whole scFv 2F3 gene into the secretive adhesion expressing vector pFUW80. 我们把整个单链抗体2F3基因插入到分泌型融合表达载体pFUW80中，建成了人源化单链抗体2F3基因库。
- Fig.1.Construction of the shuttle expression vector pRL_hEGF. 标题： 图1.;穿梭表达载体pRL_hEGF的构建。
- HLF;sperm;liposome;glandmammary special expression vectors;transgenic animals. 01人乳铁蛋白;精子;脂质体;乳腺特异表达载体;转基因动物
- The pPIC9K/Ang-1 yeast expression vector was constructed successfully. 成功构建pPIC9K/Ang-1毕氏酵母表达载体。
- Aim To construct a CHO cell expression vector carrying coamplification gene. 目的构建携带共扩增基因的CHO细胞表达载体。
- Our goal was to clone this receptorusing molecules produced in eukaryotic cells. CCL18 expressing vectors and asoluble fusion protein made of CCL18 fused to the Fc portion of human ormurine IgG1 were constructed. 构建了人CCL18及连有人或小鼠IgG1 Fc片段的CCL18的真核表达载体。
- Objective To construct the antisense nucleic acid eukaryotic expressing vector of the cDNA sequence encoding the transmembrane domain of receptor tyrosine kinase RON (RON-RTK) gene (RONm). 目的构建RON基因跨膜区段(RONm)的反义核酸真核表达载体。
- The eukaryotic expressing vector encoding human cbl is constructed and it expresses flag-cbl fused protein correctly in COS-7 cells. 成功构建了N 末端带flag标签的cbl真核表达载体 ;并使其在真核细胞COS 7中表达 .
- Cyclin D1 and CDK4 expression vectors which can express Cyclin D1 and CDK4 fusion proteins are successfully constructed. 本研究成功构建了细胞周期蛋白D1及CDK4原核表达载体,后两者在细胞内能正确表达细胞周期蛋白D1及CDK4融合蛋白。
- The recombinant plasmid pMD-ORF2 was digested with EcoR V and Xhol I and then the fragment was sub-cloned into the prokaryotic expressing vector pET-32a(+), getting the recombinant plasmid pET-ORF2. 将重组质粒pMD-ORF2的EcoR V和Xhol I双酶切产物亚克隆到原核表达载体pET-32a(+)，构建ORF2基因重组表达质粒pET-ORF2。
- Cloning of ACC Oxidase Gene of Peach and Construction of Its Plant Recombinant Expression Vectors?? 桃ACC氧化酶基因的克隆和植物表达载体的构建
- Meanwhile, expression vectors were constructed with sence and antisence of PCaM-1 cDNA. 同时构建PCaM-1的正、反义真核表达载体，以转化花生研究CaM在胚胎发育中的作用。
- The cDNA of BcpLH gene was cloned into a His-fusion expression vector pET-28a (+) and was induced to express in E. colt strain BL21(DE3). 在含有His标记序列的原核表达载体pET28-a(+)上插入Bc-pLH基因的cDNA,在大肠杆菌BL21(DE3)中诱导表达出了特异性蛋白,并免疫大白兔制备出高效价的抗血清;
- Objective To construct endothelial cell-specific molecular-1(ESM-1) eukaryotic express vector,transfect it into human umbilical vein endothelial cells( HUVECs,ECV304) and express it in vitro . 目的 构建人内皮细胞特异性分子 1(ESM 1)真核表达载体 ,转染人脐静脉内皮细胞系ECV30 4 ,并在ECV30 4中获得表达。
- And then the CED-9 gene was inserted into plant expression vector pBI121 under the control of CaMV 35S promoter. 在此基础上再构建重组表达载体pBI-ced9，将CED-9基因置于CaMV35S启动子控制之下。
- The baculovirus expression vector pAC-HBs-Fc for complete IgG antibody against HBsAg was successfully constructed. 已成功构建表达载体pAC-HBs-Fc,为表达抗人HBsAg的IgG全抗体奠定了基础。
- The cDNA was subcloned into prokaryotic expression vector pET3d and overexpressed in E. coli BL21(DE3). 将该cDNA插入原核表达载体pET3d并在大肠杆菌BL21(DE3)中过量表达。
