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- Keywords Saccharomyces cerevisiae;expression system;homologous recombination;yeast episomal plasmid;plasmid stability;plasmid copy number;fusion protein; 酿酒酵母;表达系统;同源重组;附加体型载体;质粒稳定性;质粒拷贝数;融合蛋白;
- episomal plasmids 附加体质粒
- yeast episomal plasmid 酵母附加体质粒
- yeast episomal plasmid(YEp) 酵母附加体质粒
- The plasmid pBV220-Arr. could be expressed in E. 成功构建的重组质粒pBV220-Arr在E.
- The recombinant plasmid pUC18 E6 had been got. 获得重组质粒 pUC18 E6。
- The three plasmids were effectively transferred into 293T c ells. 表达慢病毒的3种质粒可高效转染入293T细胞。
- Recombinant plasmid pGEX-Csn was transformed into E. 重组质粒pGEX-Csn转化E.
- Objective To construct the plasmid expressing BDV p40 gene. 目的构建博尔纳病病毒p40基因重组表达质粒。
- In addition, a set of expression plasmid vectors, PMS-31b. 同时构建一组质粒表达载体PMS-31b。
- Constructed the expression plasmid pTrc-rCR and checked it. 构建表达质粒pTrc-rCR,并酶切检验;
- The PNL-BDNF-IRES2-EGFP plasmid was identified to be correct. 构建的慢病毒载体质粒PNL-BDNF-IRES2-EGFP经Sal I和BamH I双酶切鉴定正确,三质粒共转染293T细胞后荧光激发可见大量绿色荧光。
- In the 6th week plasmid DNA could only be detected in quadriceps. 第6周除在股四头肌可检测到外,其余组织中均为阴性;
- I focus on the role of antisense RNAs in plasmid replication. 本文着重叙述反义RNA在质粒复制中的作用及其进展。
- The key to this riddle appeal to be plasmids, which can block phage adsorption. 这个难题的关键是质粒,它能够阻碍噬菌体的吸附作用。
- New phages can develop by acquiring restriction enzymes from plasmids. 通过从质粒获得的限制性酶,新的噬菌体能够生长。
- The plasmids enter the plant tissue and produce a swelling, or tumor. 原生质进入植物组织后产生了浮肿，或者说是肿瘤。
- Lysogenic phages have double-stranded DNA genomes, as do plasmids and episomes. 溶源性噬菌体及质粒和游离基因则含有双链DNA基因组。
- Method: One episomal HPV16 CTSE and one wild-type HPV16 CTSE fragment were amplified with PCR and inserted into pUCm-T vector respectively, following pUCm-T vector reference. 方法：本实验选取游离型HPV16阳性的宫颈癌组织（已测序证实存在变异的CTSE片段）及野生型HPV16质粒DNA为模板，PCR扩增CTSE片段，克隆到中间载体pUCm-T，酶切、PCR、测序等方法鉴定插入成功。
- The specificity of probes was tested by plasmids of the target fragments. 方法基于早期实验室研究挑选出28条寡核苷酸探针制备检测芯片。
