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- Electrophoresis mobility shift assay(EMSA)showed that both NPs of Z10 and Z37 hantaviruses bound specifically to the double-stranded DNA probe possessing the 18-nucleotide inverted repeat sequences in vitro. 然后将该探针与纯化的Z10、Z37株的核蛋白NP进行非变性凝胶电泳迁移改变实验(EMSA)后发现,重组的Z10、Z37株的核蛋白NP,在体外均可特异地结合其基因组末端反向重复序列形成的双链探针。
- Electrophoresis mobility shift assay 电泳迁移率变动分析
- The biological activity of renatured AIF protein was detected by electrophoretic mobility shift assay (EMSA) and Hoechst staining. 采用凝胶滞后实验(EMSA)与Hoechst 33258染色检测AIF蛋白的生物学活性。
- Electrophoretic mobility shift assay (EMSA) and competition tests also demonstrated its binding activity to the target element. 体外凝胶电泳阻抑分析及竞争实验亦证明该蛋白质与沉默子DNA存在相互作用。
- The changes of blood pressure, whole blood viscosity and red blood cell (RBC) electrophoretic mobility (EPM) of all the three groups were determined. 方法:将大鼠随机分成对照组、烫伤组和驻极体治疗组,分别测量对照组、烫伤组和驻极体治疗组大鼠血压、全血粘度和红细胞电泳率的变化。
- The oxidative modification of LDL was identified by agarose gel relative electrophoretic mobility(REM)and fluorescence of thiobarbituric acid reaction substances(TBARS)contents. 测定LDL的电泳迁移率及硫代巴比妥酸反应物质含量。
- The electrophoretic mobility shift assay (EMSA, gel shift, gel retardation) is a relatively simple and sensitive method to investigate protein:DNA interactions. 电泳凝胶迁移分析是一个相对简单和灵敏的研究蛋白:DNA相互作用的方法。
- This fluorescence-based Electrophoretic Mobility Shift Assay (EMSA) Kit provides a fast, easy and quantitative method to detect both nucleic acid and protein in the same gel. EMSA试剂盒提供快速、简单和定量的方法在同一块凝胶中检测核酸和蛋白,在标准的电泳凝胶迁移中获得双倍的信息。
- Under the conditions of low surface potential and weak applied electric field, the electrophoretic mobility of a particle is evaluated by a superposition method. 在低表面电位、弱外加电场与低雷诺数的假设下,吾人利用加叠原理求解相关电场与流场之线性化方程组,并获得胶体粒子的电泳速度(或电泳迁移率)。
- Particle electrophoretic mobility (PEM) changes are shown to correlate well with the amount of ligand fixed on the particles, as probed by its biological activity. 通过对配体生物活性的探测,发现粒子电泳迁移率(PEM)的变化与固定在颗粒上的配体数量密切相关。
- With the enzymes, however, one may add to the criteria of homogeneity in solubility behaviour, sedimentation, and electrophoretic mobility, the criterion of maximal enzyme activity and freedom from other enzymatic activities. 然而,对于酶而言,除了考虑溶解过程的均匀性,沉降作用及电泳淌度这些标准外, 还应该考虑最大酶活力和不参与其它酶的活动这一条标准。
- Electrophoretic mobility shift assay further indicated the HAG IL 6RE homologous to IL 6 responsive element at -568 site of the angiotensinogen promoter binds C/EBP(CAAT/Enhancer Binding Protein). 结果IL-6使血管紧张素原mRNA明显提高,位于血管紧张素原基因启动子中的HAGIL-6RE结合于转录因子CAAT/增强子结合蛋白(C/EBP);
- We measured and analyzed the electrophoretic mobility,osmotic fragility,membrane fluidity,fourier-transform infrared(FT-IR) spectrometer and viscoelastic properties of pDCs,imDCs and mDCs by microrheogical methods. 应用微观流变学研究方法对以上细胞的电泳率、渗透脆性、膜流动性和傅立叶红外光谱(FT-IR)进行测量和分析。
- Keywords: sedimentation velocity and potential, electrophoretic mobility, electric conductivity, charged composite particle, charged porous particle, arbitrary double-layer thickne. 关键词:沉降速度与电位、电泳可动度、电导度、带电复合粒子、带电多孔性粒子、任意电双层厚度。
- A private car gives a much greater degree of comfort and mobility. 私人小汽车能给人很大的舒服和机动性。
- Mobility is very important in guerrilla warfare. 机动性在游击战中至关重要。
- Jalali-Heravi, M., Garkani-Nejad, Z., “Prediction of electrophoretic mobilities of sulfonamides in capillary zone electrophoresis using artificial neural networks”, J. Chromatogr. A, 927(2001)211-218. 朱书仪。2001。利用固相萃取/毛细管电泳检测肉品中的磺胺剂。东吴大学硕士论文。
- Keywords Hantavirus;nucleocapsid protein(NP);inverted repeat sequences;electrophoresis mobility shift assay(EMSA); 关键词汉坦病毒;核蛋白(NP);反向重复序列;凝胶电泳迁移改变实验(EMSA);
- Test the probes with electrophoresis after reclaim. 探针回收后用电泳检测。
- Methods Pulse field gel electrophoresis. 方法脉冲场电泳。