- Edman degradation showed that N-terminal residue of PAP-s was He.
- The synthetic chimera polypeptide was confirmed by Edman degradation and MALDI TOF mass spectroscopy.
- The synthetic mutant was identified by Edman degradation and ESI (electrospray ionization) mass spectrometry.
合成的突变体用 Edman降解和电喷雾质谱法进行鉴定 .
- At last, the purified products will be identified with experiments of shift fiber dissolution and Edman sequencing and will be measured specific activity.
- The avarage attachment yield of protein samples to the AP-APP and DITC-APP membran e was 68.34% and 78.63% respectively, the repetitive yield for Edman degrada tion of the AP-APP membrane and DITC-APP membrane was 90.69% and 91.47% respective ly.
用上述膜载体偶联样品进行Edman降解、测序 ;结果表明 :蛋白质样品在上述两种膜载体的偶联率分别达到6 8 34%25及 78 6 3%25 ;序列分析的重复收率分别达 90 6 9%25及 91 4 7%25 .
- DPI and its analogues which were shortened at the N-terminal of theB-chain,i. e. Des B_1(Phe)-DPI,Des B_(1-2)(Phe Val)-DPI and Des B_(1-3)(Phe ValAsn)-DPI,were prepared from A_1-(MSC)-DPI by Edman degradation.
通过Edman降解方法,从A_1-(MSC)-DPI制备了B链N端缩短的DPI类似物,即:去B_1(Phe)-DPI,去B_(1-2)(Phe Val)-DPI和去B_(1-3)(Phe Val Asn)-DPI。