e. BGC823 wtp53 cells with wild type p53,BGC823 mutp53 cell with mutant p53 alleles and BGC823 vect without p53 gene,were used. Various cell phase distribution and apoptosis were analyzed by flow cytometry in 0,8 and 24h following irradiation with 4Gy.

• 方法 3 种具有不同p53 状态的人胃癌细胞系，即转染人野生型p53 基因的BGC?823?wtp53 细胞、转染人突变型p53 基因的BGC?823?mutp53 细胞和转染无p53 基因的空载质粒的BGC?823?vect 细胞，用流式细胞计分析细胞，4Gy 照射后0、8 和24 小时后各细胞时相分布和凋亡的反应。