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- Comparing with affinity capture and dual suppression PCR, AFC pipeline can have a higher (65%) SSR enrichment, avoid unreliable sequence, need lower cost per SSR locus and its procedure is much easier. 与亲和捕捉法、双抑制PCR法相比,AFC具有65%25的SSR富集效率,避免了无效序列,操作简便、成本低。
- Studies on the Establishment and Preliminary Application of Double PCR Technique for Detection of Malaria. 疟疾双重PCR检测方法的建立和初步应用研究。
- Tester cDNAs were divided into two groups and ligated to the specific adaptor 1 and adaptor 2R, and then hybridized with normal aloe vera L cDNA twice with two rounds of suppression PCR. 将实验方的双链cDNA分成两组，分别与两种不同的接头连接(adaptor 1 and adaptor 2R)。 接着与驱逐方cDNA杂交两次及两轮抑制PCR。
- Method DNA polymorphic microsatellites of macaca rhesus were analyzed using PCR technique in 20 macaca rhesuses. 方法利用聚合酶链反应(PCR)扩增技术对20只恒河猴群个体间进行了DNA多态性的分析。
- Methods Using HE stains,Niles stains,transmission electron microscope,immunohistochemistry and RT PCR technique etc. 方法采用HE染色、尼氏染色、透射电镜、免疫组化及RT-PCR技术。
- Aim: To develop a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA. 目的：消减文库构建过程中，用PCR技术快速筛选重组阳性克隆。
- Objective To study the application of PCR technique in genetic detection of Duchenne/Becker muscular dystrophy (DMD/BMD). 目的应用PCR技术检测假肥大型肌营养不良(DMD/BMD)基因缺失和杂合子。
- Objective: (1) To establish PCR reaction system that uses allele-specific primer PCR technique to detect SNP of KLOTHO gene. 目的:(1)建立使用等位基因特异性引物方法检测KLOTHO基因单核苷酸多态性的PCR反应体系。
- A specific amplified mtDNA fragment R 2 630 WA was obtained from wild abortive type (WA) male sterile cytoplasm by AP PCR technique. 应用任意单引物聚合酶链反应技术，从水稻WA 型雄性不育系的线粒体DNA 中得到一个特异的扩增片段R2－630 WA。
- METHOD The segments of mtDNA of 9 samples of deer were amplified by PCR technique with common primes and sequenced by the DNA auto sequence machine. 方法 对 9种鹿属动物的线粒体DNA进行引物扩增和序列测定 ,并与牛科、马科的DNA序列进行比较。
- Firstly, 1.4kb and 4.3kb homologous arms were amplified from the female Dorset sheep genome by the long fragment PCR technique. 首先，利用长片段PCR扩增技术从优秀绵羊Dorset品种基因组中获得了基因打靶所需的同源臂序列，长度分别为1.;374Kb和4
- Method:Using RT PCR technique,the change of bcr/abl genes in chronic myelogenous leukemia (CML) and polycythemia vera (PV) were investigated. 方法：采用逆转录多聚酶链反应（RT－PCR）技术，研究了慢性粒细胞白血病（CML）、真性红细胞增多症（PV）bcr/abl基因的变化。
- The genomic DNA was extracted from the selective cultures and the GL-7-ACA acylase gene was directly amplified by PCR technique. 从土壤的选择性培养液中提取微生物的DNA，通过PCR的方法直接扩增成功得到GL-7-ACA酰化酶基因。
- Mixed infection of the two species of Echinococcus has been confirmed in the dog by morphological observation and PCR technique. 首次证实家犬体内存在细粒棘球绦虫和多房棘球绦虫的混合感染。
- Crime scene investigators routinely use the PCR technique to detect and identify vanishingly small amounts of DNA at crime scenes. 一直以来，刑事鑑识人员都是利用聚合酶连锁反应（PCR）来侦测及辨识遗留在犯罪现场的少量DNA。
- Results DNA extracted with improved CTAB from fresh samples of Polygonatum odoratum was successfully amplified with PCR technique. 结果改良CTAB法提取得到的DNA可以很好地用于PCR扩增。
- Method Y-STR loci systems were apply to the cases in forensic science by using fluorescein labelling multiplex-polymerase PCR technique. 方法采用Y-STR荧光标记复合扩增技术,结合案例应用。
- Conclusion Mixed infection of the two species of Echinococcus has been confirmed in the dog by morphological observation and PCR technique. 结论 首次证实家犬体内存在细粒棘球绦虫和多房棘球绦虫的混合感染。
- Methods The insert gene region of TA-GVHD and specific gene region of the Y chromosome was detected using PCR technique. 方法女性患者输血前后自身对照,采取静脉血样,用PCR技术进行TA_GVHD插入基因片段及人Y染色体特异基因荧光检测。
- Objective： To develope a PCR technique for rapid screening of phenylalanine ammonia lyase(PAL) cDNA recombinant plasmid. 本研究目的是应用PCR法快速筛查插入有苯丙氨酸脱氨酶(PAL)cDNA重组阳性克隆。
