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- downstream open reading frame 下游阅读框
- Method:The fragement of Bcr abl fusion gene was amplified from K562 cells of CML by RT PCR and was cloned into the downstream of GST gene in pGEX 6P 1 vector according to the current open reading frame. 方法 :以慢性粒细胞白血病 (CML)K5 6 2细胞株总RNA作为模板 ,采用RT PCR方法扩增包含Bcr abl(b3a2 )融合位点周围的基因片段。
- CHS gene was cloned from cDNA and CHS open reading frame sequence was obtained. 获得了CHS基因ORF(Open reading frame)的cDNA序列,全长为1299bp。
- The Prl cDNA is 1550 bp and contains a 1140 bp open reading frame that is predicted to be a protein of 380 amino acids. Pr1蛋白酶基因cDNA序列长度为1550bp;含有一个1140bp的开放阅读框;编码380个氨基酸、分子量为39120、pI=8.;06的蛋白酶前体;
- There is another protein with a different open reading frame from core protein within the HCV C gene. 除了核蛋白外,在丙型肝炎病毒核基因内,尚有一蛋白产生。
- The OsPDCD5 cDNA is a 416 bp at length, harboring a complete open reading frame of 128 aa. OsPDCD5基因的cDNA全长为416 bp,编码一个128 aa的蛋白质。
- Using genome blast, MAGE-H1/Apr-1 was mapped to chromosome Xp11.22 with the open reading frame (ORF) locating in one exon. 进一步在人基因组数据库利用Blast比较的方法将MAGE-H1/Apr-1定位在Xp11.;22。
- Using the Index of Coincidence to identify Open Reading Frames. 使用偶合指数方法来定出开放阅读框架。
- Open reading frame prediction showed that SNC73 encodes a peptide identical to the constant region of an IgA molecule in the carboxyl terminus. 对SNC73序列及开放阅读框 (ORF)进行分析 ,发现其与免疫球蛋白IgA高度同源 ,为一免疫球蛋白样基因。
- In this study, the complete open reading frame of ATM cDNA was cloned into a herpes simplex virus type 1(HSV 1) amplicon vector (pTO ATM). 本研究将 ATM c DNA完整的读码框架克隆进 1型单纯疱疹病毒 (HSV-1)扩增子载体 (p TO-ATM)。
- The open reading frame consists of a series of tri-nucleotide codons which are translated into specific amino acids in the lengthening polypeptide. 由一系列三核苷酸密码子组成,多肽延伸时翻译成特定氨基酸。
- Here, we describe the screening of 10 patients who presented with 46, XY sex reversal for mutations in open reading frame (ORF) of SRY gene. 通过筛查10位中国46,XY女性性反转病人SRY基因的开放阅读框区域,探寻新的突变类型。
- Sequence analysis revealed a 2032bp cDNA containing a 93bp 5’-untranslated region, a 706bp 3’-untranslated region and a 1233bp open reading frame. 猪CTSD基因的全长cDNA为2032bp,其中5’端非翻译区93 bp,3’端非翻译区706 bp,开放阅读框(open reading frame,ORF)1233 bp 。
- The amino acid sequence is deried from the longest open reading frame (413 amino acids;one-letter code in boldface letters). 这个氨基酸序列源于最长的开放读码框(413个氨基酸;粗体字中号码为一的那个).
- It contains an open reading frame consisting 3201bp that encodes a putative protein of 1066 amino acids with a calculated molecular mass of 118.7 kDa. 新序列的开放阅读框为3102bp,它编码了1066个氨基酸,预测蛋白分子量为118.;7KD。
- According to the data of TMV MP in the GeneBank, design gene specific primer, amplified of the whole open reading frame (ORF). 根据GeneBank中TMV MP的序列设计基因特异引物,扩增出运动蛋白基因的整个开放阅读框(ORF)。
- METHODS: Based on the cDNA sequence of DCN reported by GenBank, DCN segment with open reading frame was cloned into plasmid UF1 (UF1-DCN) after DNA sequencing. 方法:根据GenBank报道的DCN基因cDNA序列,克隆含开放读码框的DCN基因片段,测序后克隆至质粒UF1(UF1·DCN),通过磷酸钙鄄DNA沉淀法共转染293细胞,产生高滴度的含DCN基因的重组腺相关病毒(rAAV·DCN)。
- The 3737 bp cDNA has an open reading frame from nucleotide 236 to nucleotide 2134, encoding a protein with a LDNPAY motif for the binding of Numb PTB domain and 4 PDZ domains. 该基因全长3737 bp,开放阅读框从236位到2134位,其编码蛋白含有4个PDZ结构域和1个与Numb蛋白的PTB结构域作用的LDNPAY基序。
- An open reading frame (ORF) is a portion of a gene's sequence that contains a sequence of bases, uninterrupted by stop sequences, that could potentially encode a protein. 开放阅读框是基因序列的一部分,包含一段可以编码蛋白的碱基序列,不能被终止子打断。
- Each open reading frame (ORF) is knocked out using a PCR-based gene deletion strategy that takes advantage of the high degree of homologous recombination in yeast. 每个开放读码框使用基于PCR基因删除策略,使用在酵母中的高度同源重组优势。