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- dna recombinant plasmid 重组质粒
- Authentication,PCR and DNA sequencing showed the recombinant plasmid of human MIA/CD-RAP was successfully constructed. 酶切电泳和DNA测序结果表明,成功地克隆了人黑素瘤M IA/CD-RAP cDNA。
- Method The recombinant plasmid pMD18-T-gG was constructed as HSV-2 DNA standard for quantitative analysis. 方法构建重组质粒 pMD18-T-gG作为标准品。
- The recombinant plasmid pUC18 E6 had been got. 获得重组质粒 pUC18 E6。
- The recombinant plasmid PsecTaq2A_AMG was analyzed by restriction endonuclease mapping and DNA sequencing. The results of sequencing were consistent with those from GenBank. 重组克隆PsecTaq2A_AMG酶谱分析与预期结果一致 ,序列测定结果与GenBank中的人釉原蛋白序列完全一致。
- The results showed the positive for PPV DNA and PUP recombinant plasmid,but negative for the control virus and PK 15.The DNA detection limit was 40pg DNA. 结果探针与猪细小病毒DNA、PUP质粒的杂交呈阳性反应,而对照病毒、PK-15呈阴性反应,探针的最低检出限量为40pgDNA。
- Recombinant plasmid pGEX-Csn was transformed into E. 重组质粒pGEX-Csn转化E.
- Restriction analysis and DNA sequencing showed that the recombinant plasmid contained the coding region of human full length MUC1 gene. Transfection experiment verified that MUC1 gene could be expressed in COS 7 cells. 酶切鉴定和序列分析证实 ;重组质粒含有人MU C- 1全长 c DNA编码序列 ;转染实验表明 MU C- 1基因能在 COS- 7细胞中表达 .
- Methods The target DNA fragments of BDV p40 gene was obtained by PCR amplification and inserted into pEGFP-N1 reporter vector. The constructed recombinant plasmid was confirmed by PCR and DNA sequencing. 方法通过PCR方法扩增获得博尔纳病病毒p40基因的完整序列,将此片段定向克隆到pEGFP-N1载体多克隆位点区,筛选重组阳性菌株,提取重组质粒,利用PCR方法和核酸序列测定验证重组质粒构建的正确性。
- Then, the DNA fragment xylR-gfp which were obtained after pGEM-xylRGFP being digested by Kpn I and Sph I , was inserted into Kcoli-B.subtilis shuttle vector pRP22, and the resulted recombinant plasmid was named at pRP22-GFP. 经SphI/KpnI双切质粒pGEM-xylRGFP后,回收片段xylR-gfp,插入穿梭载体pRP22,得到重组子pRP22-GFP。
- Dot blot hybridization with the two probes on Nitrocellulose Membranes showed the positive results for PPV DNA from different resources and PUP recombinant plasmid,but negative for the nucleic acid samples obtained from a series of control virus. 分别对不同来源的猪细小病毒DNA及PUP重组质粒于硝酸纤维素膜上打点杂交,免疫呈色后均为阳性反应,而对照的猪瘟病毒、乙型脑炎病毒、伪狂犬病毒、PK-15细胞的核酸均为阴性反应。
- DNA recombinant technology have brought innovation to the biolog y technology, it can supply the strains with strong selectivity and ability of accumulating target metabolites. 重组DNA技术使生物技术取得了彻底性的变革,它可以提供具有极强选择性和累积目的代谢物能力的菌株。
- The recombinant plasmid with the hammerhead ribozymegene was correct by digestion identification. 核酶基因重组子经酶切鉴定序列正确。
- Recombinant plasmid pDAlllS was constructed by inserting 1.7kb aprA into the Nrul site of aveD. 将1.;7kb安普霉素抗性基因aprA插入aveD的NruI位点,得pDA1118。
- Study on Expression of Recombinant Plasmid PsecTaq2A-AMG for Human Amelogenin in Human Gingival Fibroblast. 人釉原蛋白重组质粒Psec Taq2A-AMG在人牙龈成纤维细胞表达的研究
- Objective Construct a recombinant plasmid pET28a-EDA-EDB,prepare the fusion EDA-EDB protein. 目的构建重组pET28a-EDA-EDB质粒,制备重组纤维连接蛋白EDA-EDB融合蛋白。
- Methods The pCI neo KH ORF expression vector was constructed by DNA recombinant technique and was introduced into COS 7 cells and K562 cells by lipofectactin mediated DNA transfection. Expression of KH ORF mRNA was detected by RT PCR. 方法 采用DNA重组技术构建KH ORF的表达载体pCI neo KH ORF ,通过脂质体法转染COS 7细胞和K5 6 2细胞 ,RT PCR检测目的基因的表达。
- Objective To investigate the amelogenin expression of recombinant plasmid PcDNA3-AMG in COS-1 cell line. 目的研究人釉原蛋白(AMG)重组质粒PcDNA3-AMG在COS-1细胞系中的表达。
- Methods:The HIF-dependent recombinant adenovirus Ad-5HRE/hCMVmp-BCD was constructed by DNA recombinant technique. Western blot was used to detect HIF-dependent expression of bacterial cytosine deaminase (BCD). 方法借助DNA重组技术构建HIF依赖性表达的重组腺病毒载体Ad-5HRE/hCMVmp-BCD。
- A recombinant plasmid pUC-IL6 coding for IL6 was constructed by recombinant gene technique. 利用基因重组技术,构建含IL6基因的重组质粒pUC-IL6。