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- diethylaminoethyl Sephadex 交葡聚糖凝胶
- The most frequently used commercial gel is Sephadex. 商业上常用的凝胶是交联葡聚糖凝胶。
- OBJECTIVE To determine the trap efficiency of liposomes using Sephadex. 目的考察用葡聚糖凝胶法测定脂质体包封率的影响因素。
- Diethylaminoethyl hydroethylate (DEAE-E/H), a kind of macroporous weakly basic anion exchanger, was used as carrier to immobilize aminoacylase. 以弱碱性大孔阴离子树脂DEAE-E/H为载体固定化氨基酰化酶。
- FA and FC were further isolated by Sephadex G75, and FA-1, FA-2, FC-1 and FC-2 were collected. 进一步用Sephadex G75分离FA和FC,得FA-1、FA-2、FC-1和FC-2。
- We selected BSM as expression medium, and BMP was purified by Sephadex G- 25 and then de- tected by RP- HPLC. 建立了SephadexG-25分离纯化、RP-HPLC检测BMP的有效方法。
- To set up a sephadex column chromatography method for the determination of polymer impurity in cefotetan disodium. 目的建立葡聚糖凝胶色谱法测定头孢替坦二钠中聚合物的方法。
- DEAE-Sephadex A50, Sephadex G-75 and CNBr activated Sephrose 4B were purchased from Pharmacia Corp. 主要试药:DEAE-Sephadex A50、Sephadex G-75、CNBr Activa-t。
- The yield of crude HG dHG was 0.25%. Sephadex LH-20 column chromatogramphy was used to purify dHG. 以Sephadex LH-20凝胶对HG粗dHG进行进一步的分离纯化。
- It shows that the PPMC-1 is homogeneous polysaccharide through Sephadex G-200 arbitration, acryl amide gel electrophoresis arbitration. 经Sephadex G-200等方法鉴定和理化分析表明PPMC-1为均一多糖。
- A protease purified from the fresh leaves of Acer truncatum Bunge by means of Sephadex A-50, Sephadex G-200 column chromatography. 采用Sephadex A-50;Sephadex G-200柱层析的方法;从槭树科槭树属元宝枫(AcertruncatumBunge)的叶片中提取并纯化了叶蛋白酶.
- BH-1 was isolated and purified from the fermentation broth by rough fractionation and Sephadex G-100 gel chromatography. 将发酵上清液经粗分级分离和Sephadex G-100凝胶过滤,得到具有纤溶活性的柱层析产物BH-1。
- The ultrafiltration and chromatographic experiments on Sephadex G-200 showed the range and the distribution of molecular weight. 超过滤和葡聚糖凝胶G 200实验,显示了其分子量的范围和分布。
- Twoelution peaks were obtained on the Sephadex G100 column chromatography of the crude extractand the active substance was found in the first peak. 粗提液经 Sephadex G100层析后,得到了两个洗脱峰,活性物质存在于第一峰中。
- The results showed that the CM52 cation exchangers, G-25 sephadex and D101 macroporous resin all had good isolation efficiency to the compounds. 研究结果表明:CM52阳离子交换剂、G-25葡聚糖凝胶、D101大孔树脂均可对活性成分进行较为有效的分离。
- Method: The liposomes and dissociated drugs were separated by sephadex filtration,mini-column centrifugation and dialysis. 方法:分别采用凝胶过滤法、微柱离心法、透析法分离脂质体和游离药物,并进行方法学考察,优化包封率测定条件。
- Takes a sample to Sephadex - the G75 column, collects the first peak, after the freeze drying obtains the white mPEG - SOD. 取样上Sephadex-G75柱,收集第一峰,冻干后获得白色的mPEG-SOD。
- We have chosen Sephadex G-75 as gel matrix, conducted the research about refolding of MT3-MMP and realized its refolding. 4. 我们使用Sepharose G-75作为复性凝胶介质,对金属螯合层析纯化的MT3-MMP进行了柱上复性研究,较好地实现了包涵体蛋白的复性。
- The recombinant K99 and F41 fimbriae antigens expressed by E. coli RRI (pMG611) strain had been purified by heat treatment and then by Sephadex G-100 filtratiion. 本文利用加热搅拌及Sephadex G-100凝胶过滤方法,从双价重组工程菌RRI(pMG611)中分离了重组K99和F41菌毛抗原。
- The preparation and property of Sephadex gel were briefly introduced, the adsorption behavior and application in chemistry realm of Sephadex gel were reviewed. 本文简要地介绍了交联葡聚糖凝胶的制备和性质,对其吸附性能及在化学领域的应用进行了概述。