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- denaturation renaturation 变性-复性
- The denaturation and renaturation of proteins is one of those basic problems. 蛋白质的变性、复性问题是与Hofmeister效应密切相关的生化基础课题。
- The application of Hofmeister effect in recombinant product inclusion body denaturation and renaturation in biotechnology is discussed in particular. 目前重组蛋白生产过程中包含体的变性和复性问题为上述理论研究提供了广泛的应用空间。
- The ScFv immunotoxin in inclusion was refolded to form correct structure through denaturation and renaturation showed specific and cytotoxic to gastric cancer cell line MGC803. 经包涵体纯化、变性、复性,使其折叠为正确的空间结构,经检测可与胃癌细胞MGC803特异结合,并有一定的细胞毒性。
- How is retinal pigment denaturation treated? 视网膜色素变性怎样治疗?
- This paper describe the formation, isolation, denaturation and renaturation of recombinant proteins as inclusion bodies in E. coli, and summarize the most efficient ways to refold recombinant proteins. 描述了大肠杆菌异源重组蛋白质的形成、制备、变性和复性,综述了国内外变性、复性的有效方法。
- This paper describe the formation ,isolation, denaturation and renaturation of recombinant proteins as inclusion bodies in E.coli, and summarize the most efficient ways to refold recombinant proteins. 描述了大肠杆菌异源重组蛋白质的形成、制备、变性和复性,综述了国内外变性、复性的有效方法。
- This change is considered to be due to denaturation of the enzyme. 据认为这种变化是由于酶的变性作用造成的。
- Who knows case of denaturation of retinal element pigment! 谁知道视网膜素色素变性病例!
- After renaturation, AIF protein binded DNA and induced nuclear apoptosis. 经EMSA与Hoechst 33258染色实验证实:获得的AIF蛋白具有良好地与DNA结合并可诱导肿瘤细胞核凋亡的能力,凋亡率为37%25。
- DNA renaturation :Removing the denaturation factors or in proper condition the denatured DNA (ssDNA) restore native structure (dsDNA) and function. DNA变性后,生物活性丧失,但一级结构没有改变,所以在一定条件下仍可恢复双螺旋结构。
- FTIR as a Tool to Study Cold, Heat and Pressure Denaturation of Myoglobin. FTIR用于研究肌球素的冷变性、热变性、压力变性。
- Thermal denaturation of soy proteins is a pre-requisite for tofu-gel formation. 蛋白质的热变性是制作豆腐形成凝胶的前提。
- Results: Agarose electrophoresis shows there is no obvious denaturation of RNA. 结果:RNA琼脂糖凝胶电泳显示RNA没有明显降解。
- Mobile phase composition is one of the most important factors affecting the efficiency of protein renaturation in PFLC. 蛋白折叠液相色谱法(PFLC)用于变性蛋白质复性并同时纯化时对流动相组成及其洗脱条件的要求远较通常的液相色谱法高。
- This review summarizes the renaturation techniques, especially the recent developments of refolding. 本文总结了包涵体的相关复性技术,重点介绍重折叠的最新进展情况。
- After purification, the target protein further renaturation 2 - BMP via degeneration dissolving, load dialysis bag. 纯化后的的目的蛋白进一步复性,BMP-2经变性液溶解,装入透析袋内。
- Inclusion-body protein renaturation is a important procedure in the biotechnology. 包涵体蛋白质复性是生物工程下游技术中的一个重要问题。
- Nerval pathological changes has axial mutation sex first, afterwards has pith scabbard denaturation. 神经的病变先有轴突变性,继有髓鞘变性。
- The protein refolding assisted by the molecular chaperone systems is promised to improve the protein renaturation efficiency. 蛋白质复性,即如何高效地将无活性的包涵体转变成为有活性的蛋白质,已成为基因工程蛋白产业化的瓶颈。