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- cumulus cell monolayers 颗粒细胞单层
- In general idea, cumulus cell is the best nuclear donor cell for reprogramming. 一般来说,颗粒细胞作为核供体最易被核重编程。
- Methods:91 serum specimens were used to inoculate C6/36 cell monolayers for isolate dengue virus. 方法:采集临床诊断为登革热患者急性期血清91份,接种于C6/6细胞分离病毒,应用间接免疫荧光法鉴定及分型。
- Semi-cloned embryos could be produced by injecting cumulus cell nucleus into non-enucleated oocyte, which were considered a substitute for male gamete. 作者单位:中国科学院动物研究所计划生育生殖生物学国家重点实验室;
- Oocytes were divided into two groups according to their cumulus cell apoptosis rate.Structure of oocytes was observed under light microscope and transmission electron microscope. 分为凋亡率高和低的2组,用光镜和透射电镜观察卵母细胞的结构。
- The control group of oocytes, which were not injected with the donor, was also fragmented after enucleation.Most of the couplets of the parthenotes after intact cumulus cell injection were fragmented. 卵母细胞去核后不注射供体也发生碎裂,大部分的孤雌胚(不去核)在完整的卵丘细胞被注入后同样发生碎裂。
- By adding the gG and its antibody to the culture medium of the primary CEL cell monolayers, the ILTV gG function was analyzed in ILTV attachment, penetration, direct cell-to-cell spread (CTCS) and the growth curve. 经分离纯化分别得到两种融合蛋白MBP-GG和His-GG后,分别制备了抗ILTV gG蛋白的大鼠抗血清,并利用蛋白G对两种抗血清进行了纯化,获得了纯化的免疫球蛋白。
- FSH improved nuclear andcytoplasmic maturation of COC and the effects was Mediated by cumulus cells. FSH对COC中卵母细胞核与胞浆成熟均有促进作用,其作用可能是通过颗粒细胞介导的。
- Oocytes with more than 8 layers cumulus cells had lower rate of maturation and cleavage. 具有8层以上卵丘细胞的卵母细胞的成熟率和卵裂率比较低;
- In addition, Jaenisch and his co-workers did not study mice cloned from cells taken from the bodies of adults, such as fibroblasts or cumulus cells. 除此之外,杰尼西及其同事并没有研究从成鼠身上的细胞(例如纤维母细胞或是卵丘细胞)复制出来的小白鼠。
- Study on Cumulus Cell Nuclear Transfer in Porcine 猪卵丘细胞核移植研究
- So this is the best method of IVF of C57BL/6 and DBA while the oocytes surrounded by cumulus cells of CD-1,B6DF1,FVB are directly used to IVF in the tests of fresh spermatozoa. 因此,C57BL/6和DBA小鼠理想的鲜精体外受精方法为切割透明带法,而CD-1、B6DF1、FVB小鼠切割透明带卵的受精率与卵丘卵母细胞复合体的受精率均差异不显著,故此三种品系小鼠则直接用卵丘卵母细胞复合体进行鲜精体外受精法简单有效。
- A lot of follicular compartment cells, include cumulus cells, theca cells, granulosa cells cooperate with each other to facilitate the maturation of oocytes. 卵母细胞的成熟过程至少需要经过周围卵丘细胞、卵母细胞核以及核外细胞质等几个方面一系列的变化过程。
- cumulus cell nuclear transplantation 卵丘细胞核移植
- Increased leel of LM and Col protein resulted in reduced cell monolayer permeability. 高水平的层粘连蛋白和I型胶原使细胞的通透性减低。
- Five strains of mouse (CD-I, B6DF1, FVB, C57BL/6 and DBA) oocytes were removed cumulus cells, dissected partial zona-pellucida, surrounded by cumulus cells. The techniques of IVF, culture and embryos transfer were used. 对CD-1、B6DF1、FVB、C57BL/6、DBA小鼠的卵母细胞进行直接、去卵丘细胞、切割透明带法处理后,再分别进行体外受精、体外培养和胚胎移植试验。
- Cumulus cells represent this unique subpopulation of granulose cells that surrounds the oocyte, providing nutrient, metabolic precursors and signals that regulate its development and maturation. 卵丘细胞作为围绕在卵母细胞周围的颗粒细胞亚群,对于卵母细胞减数分裂阻滞、减数分裂的恢复、卵母细胞的成熟和受精都起着重要的作用。
- Cumulus cells and skin fibroblast cells was used as donor cells, and the fusion rate of reconstructed oocytes from them was 77.1%, 68.3% respectively, there was significant difference between them. 以卵丘细胞和皮肤成纤维细胞做核供体,重组卵的融合率分别为77.;1%25,68
- Other topics at the Interface between Physics and Biology are also open for studying the cytokinetic ring contraction and cell monolayer dynamics. 现可通过电子邮件将简历、科研兴趣陈述等申请材料发给有关联系人。
- The Role of Cumulus Cell in Oocyte in Vitro Maturation 卵丘细胞在卵母细胞体外成熟培养过程中的作用