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- The recombinant constitutive expression vector pGAP9K-gat was constructed by inserting the aim gene into pGAP9K. PCR拼接获得250bp的目的基因序列,将目的基因克隆于pGAP9K,获得组成型表达载体pGAP9K-gat。
- Objective. To determine the constitutive expression and production of NGF and the effect of cytokines on the expression of NGF by human IVD cells. 目的:判断NGF的结构性表达以及产生;并人类椎间盘细胞的细胞因子对于NGF表达的作用.
- The advantage of constitutive expression vectors is that we need not feed mice with nisin, which makes the experiment on orally taken viccine in LAB more easily. pNZ8037能在乳酸乳球菌、乳酸杆菌、枯草芽孢杆菌等多种革兰氏阳性菌和大肠杆菌中稳定复制、存在,是一个广泛宿主范围的质粒。
- Low constitutive expression of TnIL-21 was observed in limited tissues of healthy fish including gut, gill and gonad. Stimulation with LPS significantly induced the expression of TnIL-21 in kidney and spleen. TnIL-21仅在正常鱼体组织的鳃、肠和性腺中有少量表达,LPS刺激能诱导TnIL-21在头肾和脾脏中表达。
- The MP fragments were then fused with constitutive expression promoter CaMV 355 and pathogen - inducible promoters CHS, Pill and BG, respectively, which led to the consmiction of plant expression vectors p355-30K, pPiII-30K, pPiII-30K and pBG-30K. MP片段分别与组成型启动子CaMV35S和病原特异诱导型启动子CHS、PiII和BG体外重组,构建成植物表达载体P35S-30K,pCHS-30K,pPiII-30K和pBG-30K。 通过农杆菌LBA4404介导,分别转化含Tin-22抗性基因的番茄品种Geneva80。
- Since constitutive expression system is more likely leading to the loss of plasmid compared with inducible system,enhancing the plasmid stability of a constitutive system is more difficult. 与诱导型表达系统相比,在组成型表达系统中,由于外源蛋白的持续表达,导致细胞代谢负担加重,质粒稳定性的控制更加困难。
- cis-dominant constitutive expression 各基因之间位显性组成表达
- Using SW480 colon adenocarcinoma cells, we hypothesized that transcription determines OPN expression.METHODS: SW480 constitutively express OPN. 本研究,我们利用SW480型结肠癌细胞系,探讨了骨桥蛋白的调控机制。
- The simple perfusing device consisted of squirmy pump and sieve and so on.The purity of rat hepatocytes were identified by the constituted expression of 6-P-Gase using enzyme-chemistry. 并根据肝实质细胞能组成型表达6-磷酸葡萄糖酶这一特性,采用酶细胞化学技术鉴定肝实质细胞。
- An expression of pain flitted across her face. 她脸上闪过一种痛苦的表情。
- He always shows a doleful expression. 他总是露出忧郁的表情。
- I can think of no greater expression of defeatism. 这是我听到的最悲观的失败主义论调了。
- A constant, variable, function name, or expression. 一种常数、变量、函数名或表达式。
- She looked at me without expression. 她毫无表情地朝我看着。
- Conclusions GRP94 is constitutively expressed within specific tissues and suggest that GRP94 might play an important role in the embryogenesis. 结论GRP94存在着基础表达,可能在某些器官的发育过程中起作用。
- The scenery there was beautiful beyond expression. 那儿的风景美丽得无法形容。
- He looked at her with a solemn expression. 他表情严肃地望着她。
- There was an expression of discontent on her face. 她脸上有不满的表情。
- Ulike the constitutively expressed CD28, ICOS has to be de novo induced on the T-cell surface, while GL50 is expressed on B cells and macrophages or induced on fibroblasts etc. ICOS主要表达在活化的T细胞上,而GL50可表达在活化的B细胞、巨噬细胞或诱导表达于成纤维细胞等多种细胞表面。
- Chemokine CCL21 is a ligand of chemokine receptor (CCR)7. It is constitutively expressed at high level in secondary lymphoid organs (thymus, lymph node, spleen). 趋化因子CCL21是趋化因子受体(CCR)7的一个配体。 它在次级淋巴器官(胸腺、淋巴结、脾脏)中组成性高表达。