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- complementary DNA probe 互补DNA探针
- Complementary DNA (cDNA) sequence of Japanese sea bass (Lateolabrax japonicus) prion protein (PrP) encoding gene was cloned and characterized through RT-PCR approach. 采用RT-PCR方法分离了鲈鱼蛋白质感染因子蛋白编码基因序列,并进行了结构分析。
- Kruh GD, Cb an A, Myers K et al. Expression complementary DNA transfer establishes map as a multidrug resistance gene[J] ,Cancer Res, 1994,54:1649. 张强;施作霖;陈梓甫.;肾细胞癌对化疗药物的多药耐药机制及其逆转[J]
- ECL is widely used in biosensor, immune sensor, DNA probe, surface analysis and enzyme analysis. ECL在生物传感器研究、免疫分析和DNA探针、表面分析和酶分析中得到了广泛的应用。
- It is possible to make specific complementary DNA's (cDNA's)that can serve as molecular probes to seek out the messenger RNA's (mRNA's)of the peptide hormones. 各种有意义的联系在所有的层次上,自抽象的理性至深刻的梦幻般的情感,层出不穷并不断重新组合排列;
- Schena M,Shalon D,Davis RW,et al.Quantitative monitoring of gene expression patterns with a complementary DNA microarray[J].Science,1995,270(5235):467-470. 李梢;王永炎;季粱;等.;复杂系统意义下的中医药学及其案例研究[J]
- DNA probe technology based on nucleic acid hybridization is a normal technique in modem molecular biology. 建立在DNA杂交基础上的基因探针技术是现代分子生物学中的一种常规技术。
- Every normal protein-making gene, for instance, has a complementary DNA sequence that sits on the other side of the ladder and usually is not transcribed into RNA. 例如,每一个正常制造蛋白质的基因,就有与之互补的DNA序列座落在阶梯的另一侧,那通常不会转录成RNA。
- Abstract: Objective:To make DNA probe labelled with HRP and discuss the conditions of hybridization reactions. 摘 要: 目的:用辣根过氧化物酶(HRP)直接标记DNA探针,并对其斑点杂交条件进行探讨。
- Objective To estimate the effort between antisense RNA probe and random primed DNA probe. 摘要目的比较不同方式标记非放射性探针的原位杂交效果。
- A study on DNA fingerprints of Erhualian and Large Yorkshire pigs was carried out using bacteriophage M13mp18 single strand DNA probe. 用噬菌体M13mp18单链DNA作探针,对二花脸和大约克夏猪进行了DNA指纹的研究。
- Methods Preparing specific DNA probe by degenerate PCR, identification and screening the cypermethrin resistance of Culex pipiens pattens. 方法简并引物PCR法制备特异性DNA探针,生物素杂交检测氯氰菊酯抗性淡色库蚊。
- It can greatly promote carcinogenesis.DNA diluted alkali firer proves N-nitroso compounds induce the crosslinking between complementary DNA base pairs and initiate cell cancerization. DNA城稀释过滤法证明,N-亚硝基化合物含氮杂环化借诱发DNA互补碱对之间的交联而启动细胞的癌变。
- The latest application of fluorescence method,spectrophotometry and electrochemical method for nanoparticle tagging DNA probe were also introduced. 介绍了荧光分析法、分光光度法和电化学分析法用于纳米粒子标记DNA检测的应用及最新发展。
- Detection of pathogenic and spoilage micro-organism in foods by DNA probe has the advantages of high specificity, sensitivity, simple and time-saving. 用基因探针技术检测食品中的有害微生物具有特异性强、灵敏度高和操作简便、省时等优点。
- The Ts254 DNA probe reacted with all the 7 isolates of Trichinella spiralis ,with the DNA from other related parasites,plasmid pBR322 and muscle tissue of mouse. 但与其它相关寄生虫、质粒pBR322、昆明株小鼠肌肉DNA均未出现杂交反应,表明Ts254探针具有一定的特异性和较高的敏感性。
- A biotin-labeled DNA probe specific for hepatitis B virus(HBV)nucleotide sequences was hybridized in situ to liver tissue of 129 cases with liver disease. 应用生物素标记HBV DNA(乙肝病毒脱氧核糖核酸)作探针,对129例肝病患者肝组织作原位杂交研究。
- Methods: Biotinylated DNA probe from sorted human chromosome 15 was hybridized to the metaphase spreads of 10 cases of APL.G-banding technique was also used in these samples. 方法:以生物素标记的人类15号染色体特异的DNA为探针,对10例APL患者骨髓中期分裂相进行杂交,所有病例均进行G显带分析。
- Methods Samples of subgingival plaque were detected by 3 kinds of oligonucleotide DNA probe from 60 sites of CP patients and 10 healthy sites of 10 healthy people. 方法利用3种寡核苷酸探针对60例慢性牙周炎患者60个患病位点、10例健康人的10个健康对照位点龈下菌斑中的牙龈卟啉单胞菌、福赛类杆菌、牙密螺旋体进行检测。
- Objective To prepare DNA probe which can be used to accurately detect chromosome 21 copy number in human interphase nuclei by fluorescence in situ hybridization (FISH). 目的制备能精确检测人类间期细胞核中21号染色体拷贝数的FISH探针。