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- collagenase DNA probe 胶原蛋白酶DNA探针
- ECL is widely used in biosensor, immune sensor, DNA probe, surface analysis and enzyme analysis. ECL在生物传感器研究、免疫分析和DNA探针、表面分析和酶分析中得到了广泛的应用。
- DNA probe technology based on nucleic acid hybridization is a normal technique in modem molecular biology. 建立在DNA杂交基础上的基因探针技术是现代分子生物学中的一种常规技术。
- Abstract: Objective:To make DNA probe labelled with HRP and discuss the conditions of hybridization reactions. 摘 要: 目的:用辣根过氧化物酶(HRP)直接标记DNA探针,并对其斑点杂交条件进行探讨。
- Objective To estimate the effort between antisense RNA probe and random primed DNA probe. 摘要目的比较不同方式标记非放射性探针的原位杂交效果。
- A study on DNA fingerprints of Erhualian and Large Yorkshire pigs was carried out using bacteriophage M13mp18 single strand DNA probe. 用噬菌体M13mp18单链DNA作探针,对二花脸和大约克夏猪进行了DNA指纹的研究。
- Methods Preparing specific DNA probe by degenerate PCR, identification and screening the cypermethrin resistance of Culex pipiens pattens. 方法简并引物PCR法制备特异性DNA探针,生物素杂交检测氯氰菊酯抗性淡色库蚊。
- The latest application of fluorescence method,spectrophotometry and electrochemical method for nanoparticle tagging DNA probe were also introduced. 介绍了荧光分析法、分光光度法和电化学分析法用于纳米粒子标记DNA检测的应用及最新发展。
- Detection of pathogenic and spoilage micro-organism in foods by DNA probe has the advantages of high specificity, sensitivity, simple and time-saving. 用基因探针技术检测食品中的有害微生物具有特异性强、灵敏度高和操作简便、省时等优点。
- The Ts254 DNA probe reacted with all the 7 isolates of Trichinella spiralis ,with the DNA from other related parasites,plasmid pBR322 and muscle tissue of mouse. 但与其它相关寄生虫、质粒pBR322、昆明株小鼠肌肉DNA均未出现杂交反应,表明Ts254探针具有一定的特异性和较高的敏感性。
- A biotin-labeled DNA probe specific for hepatitis B virus(HBV)nucleotide sequences was hybridized in situ to liver tissue of 129 cases with liver disease. 应用生物素标记HBV DNA(乙肝病毒脱氧核糖核酸)作探针,对129例肝病患者肝组织作原位杂交研究。
- Methods: Biotinylated DNA probe from sorted human chromosome 15 was hybridized to the metaphase spreads of 10 cases of APL.G-banding technique was also used in these samples. 方法:以生物素标记的人类15号染色体特异的DNA为探针,对10例APL患者骨髓中期分裂相进行杂交,所有病例均进行G显带分析。
- Methods Samples of subgingival plaque were detected by 3 kinds of oligonucleotide DNA probe from 60 sites of CP patients and 10 healthy sites of 10 healthy people. 方法利用3种寡核苷酸探针对60例慢性牙周炎患者60个患病位点、10例健康人的10个健康对照位点龈下菌斑中的牙龈卟啉单胞菌、福赛类杆菌、牙密螺旋体进行检测。
- Objective To prepare DNA probe which can be used to accurately detect chromosome 21 copy number in human interphase nuclei by fluorescence in situ hybridization (FISH). 目的制备能精确检测人类间期细胞核中21号染色体拷贝数的FISH探针。
- Gene probe See DNA probe. 基因探针:参见。
- Electrophoresis mobility shift assay(EMSA)showed that both NPs of Z10 and Z37 hantaviruses bound specifically to the double-stranded DNA probe possessing the 18-nucleotide inverted repeat sequences in vitro. 然后将该探针与纯化的Z10、Z37株的核蛋白NP进行非变性凝胶电泳迁移改变实验(EMSA)后发现,重组的Z10、Z37株的核蛋白NP,在体外均可特异地结合其基因组末端反向重复序列形成的双链探针。
- Objective To prepare the cytochrome P450 specific DNA probe of cypermethroin resistant Culex pipiens pallens and apply it to detect cypermethrin resistance of Culex pipiens pattens. 目的制备淡色库蚊氯氰菊酯抗性株细胞色素P450特异性DNA探针,并用于抗性品系与敏感品系的检测。
- The HCMV DNA probe labeled with digoxin was used for 105 women with early pregnancy (including 45 cases with a history of abnormal pregnancy) to make in situ hybridization (ISH) of chorion cells. 用地高辛标记人巨细胞病毒(HCMV)DNA探针,对105例孕早期妇女(其中有异常妊娠史者45例)绒毛细胞进行原位杂交(ISH)。
- Conclusion Dot-blot hybridization with DIG-labelled TTV DNA probe can be employed to detect TTV DNA in sera samples containing relatively high titer virus,with a sensitivity lower than that of PCR. 结论以地高辛标记的TTV探针用于斑点杂交可以检出血清病毒滴度较高的TTV,其敏感度低于PCR方法。
- Using DNA probe specific for centromere region of chromosome 3,7,8,9 and 12, we have detected the lymphocyte of peripheral blood, the fresh tissue and t he paraffin section of tumor tissue. 用3、7、8、9、12号染色体着丝粒特异性DNA探针,分别对外周静脉血的淋巴细胞、新鲜实体瘤组织、瘤组织石蜡切片进行染色体检测。