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- Objective: Traditional Ames test, in vitro mammalian chromosome aberration test, single cell gel electrophoresis assay have been used widely in monitoring chemical toxicity. 目的:传统的Ames实验、体外哺乳动物细胞染色体畸变实验、单细胞凝胶电泳实验已广泛用于化学品毒性的监测,但用于综合评价化学品的遗传学毒性还不够完善。
- MATERIAL AND METHODS: Chromosome aberration test,micronucleus test of bone marrow cell and sperm shape abnormality test in mice were used in this study. 材料与方法:采用小鼠睾丸染色体畸变试验,小鼠骨髓微核实验以及小鼠精子畸形实验方法。
- The results of Ames and micronucleus tests were negative, but the result of chromosome aberration test was positive. 风湿平Ames试验、小鼠骨髓细胞微核试验结果均为阴性; CHL细胞染色体畸变试验结果为阳性,经过S9代谢活化后致突变作用降低。
- Methods The micronucleus test of bone marrow cells, chromosome aberration test of bone marrow cells, sperm shape abnormality test in mice were carried out and the results were analyzed. 方法采用小鼠骨髓细胞微核试验、小鼠骨髓细胞染色体畸变试验、小鼠精子畸形试验进行观察。
- Spermatocyte chromosome aberration test 精母细胞染色体畸变试验
- Genotoxicity study is to study the genotoxicity and thepotential carcinogenesis effect of the test article. We can make such studies as Ames test, chromosomal aberration test, micronucleus test. 遗传毒性试验是研究受试物的遗传毒性及潜在的致癌作用。本中心可开展项目。包括Ames试验、染色体畸变试验、微核试验。
- Chromosomal aberration test in vitro 体外培养染色体畸变试验
- Keywords Aconitum Carmichaeli;Cyclophosphamide;Micronucleus Test;Sister Chromatid Exchange Test;Chromosome Aberration Test;Processing of Traditional Chinese Crude Drugs; 川乌;环磷酰胺;微核实验;姊妹染色单体交换实验;染色体畸变实验;中药炮制;
- chromosome aberration test 染色体畸变试验
- Mothods: Genotoxicity and teratogenicity of DSL were examined by micronucleus of PCE,chromosome aberration of CHL and teralogenic test. 方法:采用小鼠骨髓嗜多染红细胞(PCE)微核试验、中国仓鼠肺细胞(CHL)染色体畸变试验及致畸试验,对受试物DSL进行了遗传毒性及致畸性检测。
- Methods Sperm abnormality test, Chromosome aberration of spermatogonia and unscheduled DNA synthesis (UDS) test in male mouse reproductive cells were used in the study. 方法采用小鼠精子畸形试验、精原细胞染色体畸变试验和小鼠精子非程序DNA合成(UDS)试验。
- And chromosome aberration of bone marrow cells of mice was observed. 观察小鼠骨髓细胞染色体畸变。
- Methods : Micronucleus test in bone marrow PCE and chromosome aberration assay in primary spermatocyte were undertaken in mice exposed to the immersion. 方法:用香烟烟蒂生理盐水浸泡液为实验材料进行小鼠骨髓嗜多染红细胞(PCE) 微核试验和睾丸初级精母细胞染色体畸变试验。
- Water organic pollutants induced gene mutation and chromosome aberration in abundant season were tested by Ames test and micronucleus test (MNT). 用Ames实验、微核实验检测了丰水期水样中有机污染物引起的基因突变与染色体畸变;
- Method: salmonella typhimurium mutagenicity test (Ames test), chromosome aberration (CA) test of cultivated cells of mammalian, micronucleus test in mice and mammalian bone marrow CA test were used. 方法:应用鼠伤寒沙门菌回复突变实验(Ames实验)、哺乳动物培养细胞染色体畸变(Chromosome Aberration, CA)实验、小鼠微核(Micronucleus, MN)实验和哺乳动物骨髓细胞CA实验。
- The chromosome aberration rates were 15.3% for children with mental retardation and 3.7% for control group. 智力低下儿童染色体畸变率为15·3%25,对照组为3·7%25;
- spermatogonial chromosomal aberrations test 睾丸精母细胞染色体畸变试验
- The mental retardation is correlative with the chromosome aberration and the fragile site expression rate. 提示精神发育迟滞与染色体的畸变率和脆性部位的表达率有一定的相关性。
- Objective:To study the relationship between the chromosome aberration and the male infertility. 目的研究男性染色体异常与男性不育之间的关系。
- Conclusion: The mental retardation is correlative with the chromosome aberration and the fragile site expression rate. 结论:提示精神发育迟滞与染色体的畸变率和脆性部位的表达率有一定的相关性。