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- VSMCs were lysed by cell lysis buffer and the concentration of protein was measured using Coomassie blue G250 staining technique. 裂解细胞,考马斯亮蓝G一250染色法测定蛋白浓度,使用节触stern Blotting法检测
- VSMCs were lysed by cell lysis buffer and the concentration of protein was measured using Coomassie blue G250 staining technique. ATi protein expression was determined by Western Blotting. 裂解细胞,考马斯亮蓝G一250染色法测定蛋白浓度,使用节范stem Blotting法检测ATI蛋白表达量。
- This leads to the loss of osmotic balance and ATP, and finally to cell lysis. 这会导致渗透不平衡和atp的散失,并最终导致细胞的凋亡。
- Red cell lysis is performed after the addition of the CD8-PE counter-stain. 红细胞病势渐退在CD8-PE计数器污点的添加以后执行。
- The adsorption of LCFA to cell membrane led to the cell lysis of anaerobes.Among anaerobes, acetate-utilizing ... LCFA主要通过在颗粒污泥厌氧微生物的吸附而破坏菌体细胞膜的结构,直接杀死厌氧微生物。
- However, the recovery of recombinant proteins hinges largely on the ability to effectively induce cell lysis. 然而,重组蛋白的提取有赖于诱导细胞溶解的能力。
- The mechanisms of antagonism of Trichoderma sp. including competition, mycoparasitism, antibody-secretion and cell lysis were investigated primarily. 并对木霉的竞争、重寄生、抗生、溶菌等拮抗机制进行了初步探讨和研究。
- If the cell lysis, it will release a sort of biologcal toxic substance called microcystin that will cause toxicities extensively in animal body from mammal to achordate. 铜绿微囊藻的细胞被破坏后,会释放出一种被称为“微囊体藻毒素(microcystin)”的生物活性物质,对几乎所有动物都能产生毒害作用,对昆虫也有一定的毒杀作用,因而微囊体藻毒素具备了应用于农业害虫防治的潜力。
- Combined with submerged biofilm process,ozone was used as cell lysis agent to treat excess sludge,then the ozonated supernatant was returned to the aeration tank. 采用臭氧作为剩余污泥的细胞裂解剂,并与淹没式生物膜工艺相结合进行污泥减量的实验研究,臭氧化后的污泥上清液回流入曝气池与污水合并处理。
- Methods:The proteins of HeLa cell were extracted by three different lysis buffers to do 2-DE. The gel images then were acquired by Amersham ImageMaster VDS-CL to do the comparative analysis with PDQuest(7.4.0)software. 方法:用三种不同配方的裂解液提取HeLa细胞中的蛋白质;进行双向凝胶电泳;用Amersham ImageMaster VDS-CL型凝胶成像系统获取凝胶图像;以PDQuest(7.;4
- When the cells lyse, the spores and crystals are released into the medium. 当细胞凋亡后,芽胞和结晶体释放到培养基中
- Four applications of supercritical CO_(2): extraction,cell lysis and preparation of microparticles,reaction medium,and abstersion,especially in tobacco industry are reviewed. 综述了超临界CO2的四个主要应用方向,即萃取、细胞破壁和微粒制造、反应溶剂以及SCCO2洗涤,着重总结了在烟草工业上的应用。
- Objective To set up a highly efficient cell lysis system to increase the efficiency of single cell polymerase chain reaction (PCR) for preimplantation genetic diagnosis (PGD). 目的:探讨优化细胞裂解条件,提高植入前诊断中聚合酶链反应的扩增效率。
- One inclusion formed somewhat later exterior to the exosporium. Another inclusion was formed within the exosporium and remained with the spore after mother cell lysis. 当芽胞形成细胞裂解后,一种晶体被释放出来,形成自由的伴胞晶体,另一种在芽胞外壁内侧形成,成熟后被包裹在芽胞外壁以内不脱落,始终和芽胞粘连在一起。
- In the adherent culture, the virus did not result in cell lysis after the insect cells were infected. 而在本论文中,我们尝试将此一非溶裂昆虫细胞/杆状病毒表现系统转移到旋转瓶的悬浮培养。
- Baculovirus/insect cell system has been widely used for recombinant protein production, but traditional system eventually resulted in cell lysis, so that the expressed recombinant protein was lost into medium. 摘要:杆状病毒/昆虫细胞系统已经被广泛的应用在重组蛋白质的生产上,但传统的杆状病毒感染后会造成细胞溶裂,而使得表现出的重组蛋白质流失到培养基中。
- Most of PC12 c ells treated with oxLDL showed cell lysis changes and a few cells showed apoptot ic changes, observed with electromicroscopically. 透射电镜及光镜下oxLDL作用的PC1 2细胞以坏死为主并伴少量凋亡细胞。
- About 30 minutes after infection the bacterial cell lyses and the phage particles are released. 侵染后约30分钟,细菌细胞裂解,释放出噬菌体颗粒。
- Enzymes for Molecular Biology and Cell Lysis 分子生物学酶和细胞裂解酶
- A storage cell in some amorphous memory devices. 某些非晶体存储器中的一个存储单元。