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- The investigator needs only to supply the cloned DNA containing a good E. coli promoter and ribosome binding sites. 研究者只需准备含有合适的原核启动子和核糖体结合位点的克隆DNA就行。
- Protein Expression: A Practical Approach details the expression of cloned DNA or RNA templates in all the major in vivo and in vitro systems. 蛋白质表示: 一实际方法细节克隆DNA 或者RNA 模板在全部主要方面的表示在活体内和在试管内系统。
- In this system, cloned maize mitochondrial DNA(mtDNA) is served as template. this is the first established cell-free system for in vitro transcription of cloned DNA with a plant RNA polymerase B. 这是首次用克隆化DNA为模板,以植物RNA聚合酶B建立起的无细胞离体转录系统。
- B7 1(CD80) cDNA was cloned by RT PCR from human B lymphma Raji cell line and confirmed by DNA sequencing. 应用RT-PCR技术从人B淋巴瘤细胞系Raji中克隆到B7-1(CD80)cDNA,并经测序证实。
- Complementary DNA (cDNA) sequence of Japanese sea bass (Lateolabrax japonicus) prion protein (PrP) encoding gene was cloned and characterized through RT-PCR approach. 采用RT-PCR方法分离了鲈鱼蛋白质感染因子蛋白编码基因序列,并进行了结构分析。
- METHODS: Based on the cDNA sequence of DCN reported by GenBank, DCN segment with open reading frame was cloned into plasmid UF1 (UF1-DCN) after DNA sequencing. 方法:根据GenBank报道的DCN基因cDNA序列,克隆含开放读码框的DCN基因片段,测序后克隆至质粒UF1(UF1·DCN),通过磷酸钙鄄DNA沉淀法共转染293细胞,产生高滴度的含DCN基因的重组腺相关病毒(rAAV·DCN)。
- The ORF of two circadian gene BMAL1 and CRY1 were cloned from normal human whole brain marathon cDNA and verified with restriction enzyme digestion and DNA sequencing. 从正常人全脑marathon cDNA中克隆到人生物节律基因BMAL1和CRY1的开放读码框架(ORF),经酶切和测序鉴定与GeneBank数据库中收录的序列基本一致。
- Each clone would have the same DNA. 每一个克隆人都有着同样的dna。
- A new gene APG was cloned and its cDNA full-length is 247bp. 克隆了一个肝癌凋亡相关基因,cDNA全长为563bp。
- Fast Method for Cloning DNA Big Fragments 快速克隆DNA大片段的方法
- Objective: To clone and sequence cDNA of human lymphotoxin (LT). 目的:克隆人淋巴毒素cDNA并测序。
- Characterization of A Cloned DNA Fragment of Rice Chloroplast and Location rbcL Gene in the Recombinant Plasmid 水稻叶绿体DNA克隆片段的分析和rbcL基因在重组质粒上的定位
- 3、For genomic analysis, one does not typically sequence dictly from clone DNA carried in a lambda vector. why not? 酵母人工染色体的控制系统具有三个部分:着丝粒、端粒、酵母自主复制序列。还有选择标记基因。
- CHS gene was cloned from cDNA and CHS open reading frame sequence was obtained. 获得了CHS基因ORF(Open reading frame)的cDNA序列,全长为1299bp。
- Each plasmid contains a cDNA insert that was cloned via EcoRI and XhoI. 每个质体含有一段cDNA,此插入基因是利用EcoRI与XhoI来复殖的。
- We have cloned a cDNA sequence of the human kinase SBK1(homo sapiens SH3-binding domain kinase 1)for the first time. The genomic DNA sequence of human SBK1 has been acquired through an electronic clone by the bioinformatics tools. 首次克隆到人的SBK1(homo sapiens SH3-binding domain kinase1;SBK1)的cDNA序列;并通过生物信息学的手段;电子克隆到人SBK1的基因组DNA序列.
- DNA fingerprinting can help the police to find the criminals. DNA指纹术有助於警察发现罪犯。
- METHOD:PF4 complementary deoxyribonucleic acid (cDNA) was amplified by polymer ase chain reaction (PCR), then cloned into pUC19 vector. After sequencing,PF4-c DNA was subcloned into eukaryotic expression vector pIRES2-EGFP. 方法:人工合成PF4基因模板,通过PCR方法扩增PF4-cDNA,然后克隆至pUC19克隆载体,经序列测定后重组入pIRES2-EGFP真核表达载体并进行酶切鉴定。
- Objective:To clone HLA A *0201 cDNA and express it transiently on COS 7 cells. 目的 :克隆HLA A 0 2 0 1编码区的cDNA ,并建立在COS 7细胞上的瞬时表达系统。
- Expression of the Tea cystatin cDNA in E. coli[J]. 引用该论文 王朝霞;江昌俊;余有本;房婉平.