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- biolistic transfection 生物导弹转染
- After ATF gene transfection, the ATF mRNA was obviously expressed. 结果表明,转染ATF基因后,肿瘤细胞呈现ATF基因的明显表达;
- Transfection, make stable C/EBP p expression cell line. 质粒转染,制备稳定表达C尼BPp的细胞系。
- Cell density was associated with PEI/DNA transfection efficiency. 细胞密度影响转染效率;
- Gene transfection is the key technique of gene therapy. 摘要基因治疗的关键技术是基因转移。
- Objective To construct Giardia canis virus(GCV) transfection vector. 目的构建犬贾第虫病毒(Giardia canis virus,GCV)转染载体。
- GFP can be used as a reporter system for optimizing transfection of K562 cells. 结论:电转染较脂质体转染K562细胞的效率高,GFP可作为报告系统优化K562细胞转染条件。
- The inhibitory effect of gene transfection on solid tumor was observed in mice. 小鼠实体瘤块模型研究基因转染抑制肝癌生长作用。
- Hoechst and HE staining showed that apoptosis was induced after transfection. 细胞HE染色部分细胞呈典型的凋亡细胞形态学改变;
- Combining the osg6B promoter and Cre/loxP system, we constructed a set of plasmids suitable for biolistic transformation. 以osg6B为启动子,构建了一组受控于Cre/loxP位点特异性重组系统的适用于基因枪法转化小麦的载体系统。
- Transcription activation expression vector (pAHC25ABP9) and reporter vector (pIGA) were co-transformed into Maize suspension cells by biolistic method. 以玉米悬浮细胞为受体材料,通过基因枪转化法将体内转录激活表达载体和报告质粒载体进行共转化。 实验结果证明ABP9能够与ABRE结合并启动下游报
- Positive Ion Lipoplexes and Its Application in Gene Transfection and Gene Therapy. 阳离子脂质体及其在基因转移和基因治疗中的应用。
- The study of gene transfection into rabbit's tongue muscle in vivo and optimization. 分子外科缝线术的建立及其优化方案的研究。
- RT-PCR showed that E1A gene had a stable transfection in Hela cells and obviously down-regulated Her-2 expression. RT-PCR结果显示:E1A基因在Hela细胞中能稳定表达,E1A基因的转染明显降低了Her-2基因在Hela细胞中的表达水平;
- Objective To optimize the transfection rate in COS 7 cell line with electroporation method. 目的 以电穿孔法转染COS 7细胞系以得到较高的转染率。
- Objective To study the effect of ULBP3 gene transfection on the sensitivity of Saos-2 cell line to human NK killing. 目的 观察ULBP3基因转染对人外周血NK细胞对Saos -2细胞杀伤活性的影响。
- Exogenous hTERT gene transfection could promote the proliferative capacity of hEF. 外源性hTERT基因转染可使hEF增殖能力增强。
- This article reviews about progress in gene transfection technique to myocardium i... 本文仅就目前体内心肌基因转染技术的进展作一综述。
- Methods:The plasmid pcDNA3 containing the wild type p16 gene and neo gene was used in the transfection experiment. 方法:采用pcDNA3质粒进行转染,该质粒含有野生型P16基因片段和新霉素抗性基因。
- Transfection showed an efficiency of 90% in ADAS cells. BMP-7 expression in ADAS cells was identified by Western blot. 早期对ADAS cells的转染效率可达90%25, Western-Blot方法检测到第1周与第8周的转染组细胞均有蛋白水平的表达。