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- Construction of plant binary expression vector pEASPIn consideration of the different cell membrane disruption mechanism of plant defensin and CEMA, plant binary expression vector pEASP were constructed. 构建了双价抗菌肽基因植物表达载体pEASP
- Second, five plant binary expression vectors pNAR501, pNAR502 and pNAR503 et al driven by CaMV35S constitute promoter were constructed respectively according to the structure characteristics of open reading frame of Pib gene. 其次根据Pib基因的结构特点,将Pib结构基因克隆进双元载体构建了5个植物表达载体pNAR501、pNAR502、pNAR503等,这5个载体分别由35S驱动。
- Construction of a Plant Binary Expression Vector Harboring Two T-DNAs and Two Separate Exogenous Gene Cassettes 含双边界序列植物双价表达载体的构建
- binary expression vector 双元表达载体
- 1) Three binary expression vectors were constructed: 1) p13W4H, containing loxcDNA hairpin structure controlled by endosperm specific promoter (ESP) and hpt gene; 1) 构建了三个用于转化的双元表达载体:A:p13W4H,含胚乳特异表达启动子、lox cDNA发夹结构及潮霉素选择标记基因hpt;
- Fig.1.Construction of the shuttle expression vector pRL_hEGF. 标题: 图1.;穿梭表达载体pRL_hEGF的构建。
- In this study, with the help of the intermidiate vector pJIT163,2-5A gene and Rnase L gene were cloned into the binary plant expression vector pBINplus to obtain the plant transformation/expression vector pBIN2-5A and pBINRL. 本文利用2-5A基因和RNase L基因,通过中间载体pJIT163将2-5A基因和RNase L基因克隆到双元植物表达载体pBINplus上,分别构建了2-5A基因的植物表达载体pBIN2-5A和RNase L基因的植物表达载体pBINRL。
- The pPIC9K/Ang-1 yeast expression vector was constructed successfully. 成功构建pPIC9K/Ang-1毕氏酵母表达载体。
- Aim To construct a CHO cell expression vector carrying coamplification gene. 目的构建携带共扩增基因的CHO细胞表达载体。
- A binary code in which sequential numbers are represented by binary expressions, each of which differs from the preceding expression in one place only. 一种二进制编码,其中顺序的数采用二进制表达式,每一个表达式仅在一位上和它前一个数的表达式不同。
- A binary code in which sequential numbers are represented by binary expressions,each of which differs from the preceding expression in one place only. 一种二进制编码,其中顺序的数采用二进制表达式,每一个表达式仅在一位上和它前一个数的表达式不同。
- And then the CED-9 gene was inserted into plant expression vector pBI121 under the control of CaMV 35S promoter. 在此基础上再构建重组表达载体pBI-ced9,将CED-9基因置于CaMV35S启动子控制之下。
- The baculovirus expression vector pAC-HBs-Fc for complete IgG antibody against HBsAg was successfully constructed. 已成功构建表达载体pAC-HBs-Fc,为表达抗人HBsAg的IgG全抗体奠定了基础。
- The cDNA was subcloned into prokaryotic expression vector pET3d and overexpressed in E. coli BL21(DE3). 将该cDNA插入原核表达载体pET3d并在大肠杆菌BL21(DE3)中过量表达。
- Objective To construct the antisense expression vector of human Na + H + exchanger 1 (NHE 1). 目的 构建人Na+ H+ 交换蛋白 1(Na+ H+ exchanger 1,NHE 1)基因反义真核表达载体。
- Cloning of recombinant human BMP2 gene in eukaryotic expression vector provide basis for BMP2's expression. 克隆获得人骨形成蛋白 2基因 ,并得到此基因的真核表达载体 ,为人骨形成蛋白 2的表达打下了基础。
- Objective:To construct expression vector of human ID4 gene promoter on the basis of bioinformatics analysis. 目的:基于生物信息学分析构建人ID4基因启动子表达载体,以其作为研究ID4基因启动子表达调控分析的工作基础。
- The recombinant constitutive expression vector pGAP9K-gat was constructed by inserting the aim gene into pGAP9K. PCR拼接获得250bp的目的基因序列,将目的基因克隆于pGAP9K,获得组成型表达载体pGAP9K-gat。
- The competent Agrobacterium tumefacien was transformed by rice expression vector p13W4-CTB and pCWUN respectively. 水稻表达载体P13w4一cTB和pcWIJN分别转化感受态根瘤土壤杆菌,经过PcR筛选和酶切鉴定,获得根瘤土壤杆菌转化子。
- For mass production of hEGF, Bombyx mori baculovirus expression vector system was adopted in this experiment. 为了高效表达人表皮生长因子,我们采用了家蚕杆状病毒表达系统(Bombyx mori baculovirus expression vector system, BMBEVS)。