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- 以碘化丙錠(propidium iodide,PI)結合流式細胞術分析細胞周期分佈情況,AnnexinV-APC/CFSE雙染結合流式細胞術並使用DNA Ladder凋亡檢測試劑盒分析細胞凋亡的情況。Distribution of cell cycle was analyzed by propidium iodide (PI) staining associated with flow cytometry. Cell apoptosis was analyzed by AnnexinV-APC/CFSE double stainging, flow cytometry and by method of DNA Ladder.
- 方法:採用Daniel方法建立神經元缺氧凋亡模型,加入補陽還五湯藥物血清,應用碘化丙啶(propidium iodide,PI)染色法經流式細胞儀檢測神經元凋亡率,免疫組化法流式細胞儀檢測p53和p21的表達。Methods: The model of hypoxia neuron apoptosis was established adopting Daniel method and treated with BHD drug serum. The neuron apoptosis rate was determined by flow cytometry with propidium iodide staining, the p53 and p21 gene expression was tested by immunohistochemical method with flow cytometry.
- 方法將燒傷血清和痂下水腫液與人臍靜脈內皮細胞一同孵育12、24 h后,採用Annexin-V-Fluos(A-V)和碘化丙啶 (Propidium iodide,PI)標記內皮細胞,流式細胞儀檢測其凋亡和壞死百分率。Methods Human umbilic vein ECs cultured in vitro and stained with annexin-V-Fluos (A-V) and propidium iodide (PI) were incubated with peripheral blood serum or STF collected from patients with severe burn injuries, and apoptosis and necrosis of the EC were observed by means of flow cytometry.