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- The Restriction Enzyme Database from NEB. 限制苺資料庫取自新英格蘭生物實驗室公司。
- It enables a specific gene to be located on a particular restriction enzyme fragment. 它就能使專一的基因被定位於特定的限制性內切酶切成的片段上。
- Based on the number of restriction enzyme detecting RFLPs, most of mutations were attributed to point mutation. 根據揭示多態性的限制性內切酶的數量可將產生的突變大多歸為點突變。
- Certain DNA sequences oriented within this gap are substrates for Alu I, a blunt end restriction enzyme. 如果用一段酶切斷作為橋樑的DNA,那麼整個電路的電流也被中斷了。
- Amplified of the green fluent protein (GFP) gene from plasmid PCR3.1, and add suitable restriction enzyme cutting site. 從含有gfp基因的質粒pCR3.;1上擴增出全長基因,並且加上合適的酶切位點。
- Results: Recombinant expression plasmid of ALR was confirmed correct by restriction enzyme digestion and sequencing. 結果:酶切鑒定及測序結果提示表達產物正確;
- The expressive vector pcDNA3.1(-)-ASL has been constructed and had been confirmed by restriction enzyme cut and sequencing. 構建的真核表達載體pcDNA3 .;1(-) ASL經過酶切鑒定和測序證實正確。
- Genomic DNA was extracted.Polymerase chain reaction, direct sequencing and restriction enzyme reaction were performed to identify the mutations. 利用聚合酶鏈反應、DNA直接測序進行突變檢測,進一步採用限制性內切酶酶切驗證突變。
- Restriction enzyme digestion and AT clone were employed to rapidly screen out the recons of PA segments for the preparation of the gene chip probes. 利用酶切、AT克隆方法快速分析篩選出保護性抗原基因片段的重組子,從而製備成晶元探針。
- The vector was identified via PCR and restriction enzyme analysis and was introduced into Agrobacterium tumerifacience LBA4404. 經限制性內切酶分析和PCR鑒定后利用凍融法將重組質粒pBI121-A導入根癌農桿菌LBA4404。以錦橙 (Citrus.
- The distribution of polymorphism at restriction enzyme site-48 A/ G was analyzed with the method of polymerase chain reaction-restriction fragment length polymorphism( PCR-RFLP). 用聚合酶鏈反應-限制性片段長度多態性方法,分析限制性酶切位點-48A/的多態分佈。
- Methods: The serum samples were collected from 56 patients with morbilliform maculopapular eruption.The target DNA of CBV was detected by RT-PCR and restriction enzyme analysis. 方法:取56例春季流行性麻疹樣發疹患者外周血標本,擴增目的DNA並進行特定限制性內切酶分析。
- Results 1. The result of restriction enzyme digest and gene sequence showed that the EWS-FLI-1 binding sequence (ACCGGAAGT) lay at the upper stream of diphtheria toxin A fragment. 結果 1、對重組載體的酶切鑒定和測序結果證實,在pS2-DTA中EWS-FLI-1特異性結合序列(ACCGGAAGT)位於白喉毒素A鏈基因上游。
- The ORF of two circadian gene BMAL1 and CRY1 were cloned from normal human whole brain marathon cDNA and verified with restriction enzyme digestion and DNA sequencing. 從正常人全腦marathon cDNA中克隆到人生物節律基因BMAL1和CRY1的開放讀碼框架(ORF),經酶切和測序鑒定與GeneBank資料庫中收錄的序列基本一致。
- Restriction endonuclease (restriction enzyme) A type of enzyme, found mainly in bacteria, that can cleave and fragment DNA internally(see endonuclease). 限制性內切酶(限制酶):主要在細菌中存在的一種酶類,它可以從DNA的內部將其切開和分裂。
- Methods Polymerase chain reaction with restrict enzyme digestion was used to detect the target gene polymorphism in 78 normotension controls and 72 EH patients. 目前認為,高血壓是多種遺傳因素和環境因素共同作用而引起的複雜性狀疾病,在人類遺傳易感性的基礎上,在環境危險因素的作用下發病。
- New phages can develop by acquiring restriction enzymes from plasmids. 通過從質粒獲得的限制性酶,新的噬菌體能夠生長。
- Cut outside restricted enzymatic what be? 限制性外切酶是什麼呀?
- Restriction enzymes and reverse transcriptase are keys to gene engineering. 限制性酶和逆轉錄酶成為基因工程的重要手段。
- Obtaining of SPCEMA(signal peptide modified CEMA)SPCEMA(187bp) was amplified with two long complementary primers (P2 and P3) and two primers (Pl and P4) containing restriction enzyme recognition site. 以兩條部分重疊長鏈引物P_2和P_3延伸產物為模板,P_1和P_4為正、反引物進行PCR擴增,獲得了改造的抗菌肽基因SPCEMA(187bp)。