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Objective: To construct a procaryotic expression vector of vascularendothelial growth factor 165 (VEGF165) and to express VEGF165 inE. coli. 目的:構建人血管內皮生長因子 165(Vascular endothelial growthfactor 165,VEGF165)原核表達載體並在大腸桿菌中誘導表達。
For the first time to recombine ALR procaryotic expression vector, and get the pET28a-hALR recombined expressing plasmid which was tested by sequence analysis. 首次構建了重組ALR原核表達載體，獲得了pET28a-hALR的重組表達質粒，並測序證實。
Keywords FHIT gene;Human NIT1 gene;Procaryotic expression vector;Expression of protein;Metal chelate affinity chromatography;Antibody; FHIT基因;人NIT1基因;原核表達載體;蛋白表達;親合層析;抗體;
Fig.1.Construction of the shuttle expression vector pRL_hEGF. 標題：圖1.;穿梭表達載體pRL_hEGF的構建。
Objective To obtain the gene of human Chondromodulin-I(CHM-I) gene and express and purify it in procaryotic expression system. 目的克隆人軟骨調節素I(CHM-I)cDNA,並在原核表達系統中表達和純化。
The pPIC9K/Ang-1 yeast expression vector was constructed successfully. 成功構建pPIC9K/Ang-1畢氏酵母表達載體。
Aim To construct a CHO cell expression vector carrying coamplification gene. 目的構建攜帶共擴增基因的CHO細胞表達載體。
The more effective CHM-I clone with high purity could be expressed by procaryotic expression system. 原核表達系統可有效克隆較高純度的CHM-I基因。
And then the CED-9 gene was inserted into plant expression vector pBI121 under the control of CaMV 35S promoter. 在此基礎上再構建重組表達載體pBI-ced9，將CED-9基因置於CaMV35S啟動子控制之下。
The baculovirus expression vector pAC-HBs-Fc for complete IgG antibody against HBsAg was successfully constructed. 已成功構建表達載體pAC-HBs-Fc,為表達抗人HBsAg的IgG全抗體奠定了基礎。
The cDNA was subcloned into prokaryotic expression vector pET3d and overexpressed in E. coli BL21(DE3). 將該cDNA插入原核表達載體pET3d並在大腸桿菌BL21(DE3)中過量表達。
Objective To construct the antisense expression vector of human Na + H + exchanger 1 (NHE 1). 目的構建人Na+ H+ 交換蛋白 1(Na+ H+ exchanger 1,NHE 1)基因反義真核表達載體。
Cloning of recombinant human BMP2 gene in eukaryotic expression vector provide basis for BMP2's expression. 克隆獲得人骨形成蛋白 2基因 ,並得到此基因的真核表達載體 ,為人骨形成蛋白 2的表達打下了基礎。
Objective:To construct expression vector of human ID4 gene promoter on the basis of bioinformatics analysis. 目的:基於生物信息學分析構建人ID4基因啟動子表達載體,以其作為研究ID4基因啟動子表達調控分析的工作基礎。
The recombinant constitutive expression vector pGAP9K-gat was constructed by inserting the aim gene into pGAP9K. PCR拼接獲得250bp的目的基因序列,將目的基因克隆於pGAP9K,獲得組成型表達載體pGAP9K-gat。
The competent Agrobacterium tumefacien was transformed by rice expression vector p13W4-CTB and pCWUN respectively. 水稻表達載體P13w4一cTB和pcWIJN分別轉化感受態根瘤土壤桿菌，經過PcR篩選和酶切鑒定，獲得根瘤土壤桿菌轉化子。
For mass production of hEGF, Bombyx mori baculovirus expression vector system was adopted in this experiment. 為了高效表達人表皮生長因子，我們採用了家蠶桿狀病毒表達系統(Bombyx mori baculovirus expression vector system, BMBEVS)。
The BADH and CMO ORF were obtained and the plant expression vector pCU1303-BADH, pCU1303-CMO were constructed. 設計帶特定酶切位點的特異引物,分別克隆了麥冬BADH和CMO的編碼區片段,構建了植物表達載體pCU1303-BADH和pCU1303-CMO。
Both genes were cloned into GFP expression vector pIRES2 EGFP and transfected into HeLa cells. 將上述基因克隆入綠色熒光蛋白(GFP)表達載體pIRES2-EGFP中,轉染人HeLa細胞,在熒光顯微鏡和電鏡下觀察轉染細胞的形態和結構。
The ORF was inserted into expression vector pSE-380, then transformed into E. coli JM109 and E. 將此ORF連接入質粒pSE-380中，並在大腸桿菌JM109和BL21中表達。