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- Oridonin is the the main antitumor constituents of Donglingcao. 冬凌草甲素是中草藥冬凌草抗腫瘤的主要活性成分。
- Abstract：ObjectiveTo determine the optimal extracting process of oridonin. 目的確定冬凌草中冬凌草甲素的最佳提取工藝。
- Content of oridonin in Rabdosia rubescens was 0.6152%, and the average recovery was 97.86% with RSD of 2.14%. 測得冬凌草葉中冬凌草甲素含量為0.;6152%25，平均回收率為97
- RESULTS Content of oridonin in Rabdosia rubescens was 0.6152%, and the average recovery was 97.86% with RSD of 2.14%. 結果測得冬凌草葉中冬凌草甲素含量為0.;6152%25，平均回收率為97
- RESULTS Content of oridonin in Rabdosia rubescens was 0.6152%,and the average recovery was 97.86% with RSD of 2.14%. 結果測得冬凌草葉中冬凌草甲素含量為0.;6152%25;平均回收率為97
- Abstract: OBJECTIVE To extract oridonin from Rabdosia rubescens and determine the content of oridonin. 文章摘要: 目的 從冬凌草中提取冬凌草甲素並測定其含量。
- After recrystallizing the oridonin content is determined by TIC Scanning to be as high as 99.28%. 經重結晶後用薄層掃描法測定含量達99.;28%25;是一種簡便易行的方法。
- The oridonin contents can be controlled by control the amout of the leaves in Blushred Rabdosia. 建議通過控制藥材的含葉量來控制冬凌草片中冬凌草甲素的含量。
- Methods: It was studied by orthogonal experimental design and oridonin in extraction was determined by HPLC. 方法：通過正交試驗，以HPLC法測定提取物中冬凌草甲素的含量為指標進行研究。
- OBJECTIVE To extract oridonin from Rabdosia rubescens and determine the content of oridonin. 摘要目的冬凌草中提取冬凌草甲素並測定其含量。
- After recrystallizing the oridonin content is determined by TLC Scanning to be as high as 99.28%. 經重結晶後用薄層掃描法測定含量達99.;28%25;是一種簡便易行的方法。
- METHODS Microwave technology was used to extract oridonin and the content of oridonin was determined by HPLC. 方法採用微波萃取技術提取,HPLC法測定。
- In the process of large-scale extracting research of oridonin, a large amount of components of pigment and fat were also extracted as impurities. 在進行冬凌草規模化提取研究中,提取冬凌草甲素的同時還會夾雜大量色素及油脂類雜質,嚴重干擾冬凌草甲素的分離純化。
- Results:Oridonin could decrease the activity of telomerase, as well as cause apoptosis and inhibit the growth of K562 cells significantly. 並呈現出明顯的量-效與時-效關係。
- We assume that the structural modification of oridonin would produce novel drug candidates with improved solubility and higher potency than oridonin. 本文試從藥理作用、構效關係及其衍生物等方面對冬凌草甲素的研究概況作一綜述。
- Objective: To investigate the change of Bcl-2 expression and telomerase during the apoptosis induced by oridonin on human hepatocelluar carcinoma BEL7402 cells. 目的:探討冬凌草甲素誘導人肝癌細胞BEL-7402凋亡中Bc l-2表達及端粒酶活性的變化。
- Oridonin is an ent-kaurene diterpenoid compound mainly isolated from the leaves of R.rubescen.Oridonin has been proved to possess good antitumor activity. 冬凌草甲素是從冬凌草等植物葉子中提取出的一種貝殼杉烯二萜類化合物,具有好的抗腫瘤活性。
- By means of liqid scintillation count technique the effect of oridonin on the incorporation of [~3H] TdR, [~3H] UR and [~3H] leucine into the ECA cells in vitro was studied. 採用[~3H]TdR、[~3H]UR和[~3H]leucine在體外參入ECA細胞的液體閃爍計數術,探討冬凌草甲素對DNA、RNA和蛋白質合成的影響。
- Methods:The variation of both morphology and inhibitory rate of K562 cells were observed in culture medium with various concentrations of Oridonin at different time in vitro. 方法:以不同濃度的冬凌草甲素作用於體外培養的K562細胞,檢測細胞生長抑制率,觀察細胞凋亡時的形態學變化,對細胞凋亡前後的端粒酶活性進行檢測。
- The separation and prepartion methods of oridonin by medium pressure colunm chromatography have the advantages of fast speed,high efficiency,harmlessness,safety and economy. 採用中壓柱層析法提取分離冬凌草甲素,具有洗脫速度快,分離效率高,溶劑無毒無污染,安全、價廉、易得等優點。
