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- A coat protein gene eDNA of a PLRV isolate infected commercial potato cultivar "Purple Flower White" was synthesized by reverse transcription followed by Polymerase Chain Reaction amplification with synthesized 20merand 30mer primers. 以分離自馬鈴薯主栽品種「紫花白」的馬鈴薯卷葉病毒(PLRV)分離物的RNA為模板,用人工合成的引物,用反轉錄和隨後PCR擴增的方法合成了PLRV外殼蛋白(CP)基因的cDNA,並克隆於pUC19中。
- The coat protein gene of GPV, a Chinese serotype isolate of BYDV, was used to construct three plant expression vectors, pPPI 2, pPPI 3 and pPPI 5, which contain Act promoter or Emu promoter respectively. 以我國特有的大麥黃矮病毒 GPV株系的外殼蛋白 ( CP)基因為材料 ,設計合成了分別含有 Act啟動子或 Emu啟動子的植物表達載體 p PPI2、p PPI3和 p PPI5。
- Peanut stripe virus-Hongan isolate was obtained from the infected peanut seedling in Hongan County,Hubei Province. Its coat protein gene was amplified by RT-PCR and cloned into pGEM-T Easy vector. 從湖北省紅安縣花生種傳苗中得到花生條紋病毒(Peanut stripe virus,PStV)分離物(PStV-Hongan),提取感病葉片的總RNA,RT-PCR擴增了其外殼蛋白基因(cp),克隆至pGEM-T Easy載體,轉化大腸桿菌並鑒定。
- CDNA cloning and sequence analysis of coat protein genes of TMV and CMV isolated from tobacco in Yunnan province. 雲南省煙草花葉病毒和黃瓜花葉病毒外殼蛋白基因克隆及序列分析。
- Keywords condyloma acuminata;human papillomavirus;peripheral blood mononuclear cell;late gene;major coat protein;baculovirus expression system;enzyme-linked immunosorbent assay; 尖銳濕疣;人乳頭瘤病毒;外周血單一核細胞;晚期基因;主要衣殼蛋白;昆蟲-桿狀病毒表達系統;酶聯免疫吸附法;
- The specific primers were designed to amplify the coat protein genes of lily symptomless virus(LSV,genus Carlavirus),lily mottle virus(LMoV,genus Potyvirus) and lily virus X(LVX,genus Potexvirus) respectively. 設計3對表達引物分別擴增侵染了百合無症病毒(lily symptomless virus,LSV),百合斑駁病毒(lily mottle virus,LMoV)和百合X病毒(lily virus X,LVX)的外殼蛋白基因,並在大腸桿菌BL21(DE3)plysS中原核表達。
- Cloning of Coat Protein Gene of Maize Dwarf Mosaic Virus 玉米矮花葉病毒外殼蛋白基因的克隆研究
- Coat protein gene analysis of a strong strain of cucumber mosaic virus 黃瓜花葉病毒強毒株外殼蛋白基因克隆和序列分析
- Cloning and sequencing of the coat protein gene of Potato virus A from Yunnan 馬鈴薯A病毒雲南分離物外殼蛋白基因的克隆與序列分析
- The Integration and Transcription of Virus Coat Protein Gene in Potato Genome 病毒外殼蛋白基因在馬鈴薯基因組中的整合與轉錄表達
- Expressing Pattern of Coat Protein Gene Promoter from Cotton Leaf Curl Virus 棉花曲葉病毒外殼蛋白基因啟動子的表達方式
- Expression of Apple Chlorotic Leaf Spot Virus Coat Protein Gene in E. Coli 蘋果褪綠葉斑病毒庫爾勒香梨分離物外殼蛋白基因在大腸桿菌中的表達
- CLONING AND EXPRESSION OF PAPAYA RINGSPOT VIRUS COAT PROTEIN GENE IN E.COLI 番木瓜環斑病毒外殼蛋白基因的克隆和在大腸桿菌中的表達
- CLONING AND EXPRESSION OF TWO GARLIC VIRUS COAT PROTEIN GENES 兩株大蒜病毒外殼蛋白基因的克隆及其在大腸桿菌中的表達
- Comparision of the Coat Protein Gene Sequnce of Tobacco mosaic virus Isolated from Different Regions in Yunnan Province 雲南五個不同地區煙草花葉病毒外殼蛋白基因的序列比較
- Construction of Plant Expression Vector of the Coat Protein Gene of Zucchini yellow mosaic virus from China 小西葫蘆黃花葉病毒外殼蛋白基因植物表達載體的構建
- Yeast Expression Vectors'Construction of Barley Yellow Dwarf Virus Coat Protein Gene and Movement Protein Gene 大麥黃矮病毒外殼蛋白基因和運動蛋白基因酵母表達載體的構建
- Sequence Analysis to Coat Protein Gene of Kuerle Pear Isolation of Apple Chlorotic Leaf Spot Virus 蘋果褪綠葉斑病毒庫爾勒香梨分離物外殼蛋白基因的序列分析
- Cloning and reconstruction of expression vector of the banana mosaic virus coat protein gene 香蕉花葉病毒外殼蛋白基因克隆及表達載體的構建
- Cloning, prokaryotic expression of Cucumber mosaic virus coat protein gene and antiserum preparation 黃瓜花葉病毒外殼蛋白基因的克隆、原核表達及抗血清製備
