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- Isoelectric focusing may be used as another indication. 等電聚焦可以用為另一指標。
- Methods: Isoelectric focusing in polyacylamide gels was used. 用薄層聚丙烯醯胺凝膠等電聚焦的方法。
- After quantification, the protein samples were separated by isoelectric focusing electrophoresis and then by SDS-PAGE. 定量后，分別進行一維等電聚焦分離和二維聚丙烯醯胺凝膠電泳分離。
- Isoelectric focusing A technique used in ELECTROPHORESIS to separate amphoteric molecules (able to combine with either acids or bases). 等電點聚焦：是一種利用電泳技術分離兩性分子（能與酸或鹼結合）的技術。
- The variant(s) most likely to show pI values different from that of HbA when run on an isoelectric focusing gel. 在跑IEF時最有可能表現出與HbA不同pI的變種。
- Simultaneous Isoelectric Focusing of EsD EAP ADA on Uitrathin-layer Polyacrylamide Gels. 非平衡超薄層等電聚焦同步檢測EsD,EAP,ADA同功酶
- UTLIEF(Ultrathin-layer isoelectric focusing) was used for isozyme analysis of root-knot nematodes for the first time. 3． 首次應用超薄等電聚焦電泳技術對30個根結線蟲種群酯酶同功酶進行成功分析。
- Whitmore.Electrophoretic and isoelectric focusing technique in fisheries management[M].Boston:CPC Press,1990.28-30. 胡能書;萬賢國同工酶技術及其應用[M].;長沙:湖南科學技術出版社;198573-85
- After being concentrated and desalted, the samples were separated by isoelectric focusing on first dimension and SDS electrophoresis on second dimension. 樣品濃縮除鹽后，進行一維等電聚焦分離和二維SDS電泳分離提取人腎組織磷酸化蛋白。
- Methods:ALP isoenzymes in the serum of normal persons and tumor patients had been separated by agarose isoelectric focusing(IEF). 方法:採用神經氨酸苷酶對正常人及患者血清中ALP同工酶進行處理後進行瓊脂糖等電聚焦。
- The component and isoelectric point of all parts of the sample were deter- mined by capillary isoelectric focusing (CIEF). 經毛細管等電聚焦電泳(CIEF)的方法測定其組成及各組分的等電點。
- Methods The protein was purified by common polyacrylamide gel electrophoresis (PAGE), isoelectric focusing electrophoresis (IEF) and isotachophoresis (ITP). 方法用普通聚丙烯醯胺凝膠提純電泳(PAGE)、等電聚焦提純電泳(IEF)和等速提純電泳(ITP)對樣品進行分離。
- Methods:In CSF and serum from 398 patients with neurological diseases ,51 of whom had MS,OCB were detected using isoelectric focusing with immunofixation. 方法:收集398例神經系統疾患的腦脊液和血清標本,其中51例MS患者,用等電聚焦加免疫標記的方法進行OCB的檢測。
- RESULTS: Compared with the recommended condition, the profiles were improved by enhancing the voltage of isoelectric focusing, extending the time and desalinizing the samples. 結果： 提高聚焦電壓增強聚焦及脫鹽處理可獲得比推薦條件更好的雙向電泳圖譜。
- In this study, isoelectric focusing electrophoresis program was optimized, and various sample preparation methods, different loading quantities and strip length were studied. 在實驗中，優化了等電聚焦程序，研究了精子蛋白的不同製備方法、不同上樣量、不同膠條長度對電泳結果的影響。
- The separation modes include capillary zone electrophoresis(CZE),micellar electrokinetic chroma-tography(MEKC),isoelectric focusing(IEF) and open channel electrochromatography(OCEC). 分離模式包括毛細管區帶電泳(CZE),膠束電動色譜(MEKC),等電聚集(IEF),開管電色譜(OCEC)等。
- Esterase isozyme upon isoelectric focusing eletrophores demonstrated that the high levels of total esterase activity in ABM R was due to the increased activities of E7, E13 and E15 isozymes. 從酯酶同工酶等電聚焦電泳得出 ,ABM- R種群的 E7、E13和 E15同工酶活力顯著提高是導致 ABM- R種群酯酶活力提高的主要原因。
- On line coupling of capillary isoelectric focusing (CIEF) to electrospray ionization mass spectrometry (ESI MS) as a two dimensional separation/analysis system was employed for high resolution analysis of the protein isoforms observed during CIEF process. 在線的毛細管等電聚焦 電噴霧質譜聯用 ,作為一種二維的分離系統 ,對毛細管等電聚焦過程中形成的蛋白質亞型進行了分析。
- The separation modes include capillary zone electrophoresis(CZE), micellar electrokinetic chromatography (MEKC), isoelectric focusing (IEF) and open channel electrochromatography (OCEC). 分離模式包括毛細管區帶電泳(CZE)，膠束電動色譜(MEKC)，等電聚集(IEF)，開管電色譜(OCEC)等。
- The 16 given wild rice species belong to the genomes AA, BB, CC, CCDD, BBCC, EE, or FF. The results of esterase isozymes isoelectric focusing electrophoresis indicate that there was individually a special pattern of esterase among Ser. sativae, Ser. 對16個野生稻種(基因組分別為AA、BB、CC、CCDD、BBCC、EE和FF)的酯酶等電聚焦電泳分析表明:普通野生稻組的普通野生稻系和寬葉野生稻系、狹葉野生稻組的酯酶酶譜各有其特定模式,這和傳統的稻屬分類結果相一致。
