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- Detection of keratin 5 gene point mutation in a family with Weber Cockayne EBS by PCR and direct DNA sequencing. PCR-DNA直接測序檢測1例單純型大皰性表皮鬆解症Weber-Cockayne亞型(WC-EBS)患者角蛋白K5基因點突變
- In contrast, direct DNA sequencing requires only a single sample from the proband. 相比之下,直接測序只需要被檢測者的血樣 。
- Methods:ABO blood groups were identified by serological tests. B(A) alleles were determined by PCR-SSP and direct DNA sequencing at exons 6 and 7 of ABO gene. 方法:用血清學血型方法、PCR-SSP法和ABO基因第6及第7外顯子直接測序的方法對B(A)血型和B(A)型等位基因進行檢測。
- The family was blood-grouped serologically as AB phenotypes,while PCR-SSP assay showed as O/O phenotypes,but direct DNA sequencing at exons 6 and 7 of ABO gene indicated that they were B(A)/O phenotypes. 該家系中血型血清學定型為AB型的成員DNA序列分析測定基因型為B(A)/O型,而PCR-SSP法檢測AB型成員的基因型為O/O型。
- Method: PCR amplification,DNA sequencing and cladistic analysis. 方法:PCR擴增、DNA序列測定、分支分析。
- HBeBP4A gene was successfully amplified and identified by DNA sequencing. 成功擴增出HBeBP4A基因,測序結果符合GenBank報告序列。
- Laboratory diagnosis of Hgb Hekinan using HPLC and DNA sequencing methods? 施浤彰(Hung-Chang Shih);施木青(Mu-Chin Shih);張煜昌(Yu-Chang Chang);林惠茹(Hui-Ju Lin);林彩秀(Tsai-Hsiu Lin);彭慶添(Ching-Tien Peng);張天傑(Tien-Jye Chang);張建國(Jan-Gowth Chang)*?
- The methylation specific PCR (MSP) and bisulfite DNA sequencing were performed to examine the methylation status of SLIT2 gene promoter. 亞硫酸氫鈉處理DNA,用MSP和測序法檢測SLIT2啟動子甲基化狀態。
- New primers were designed for hot - start PCR reaction according to the results of DNA sequencing. 根據測序結果設計新的引物以進行熱啟動PCR反應;
- It was proved that the E2 genes were integrated stably into chromosome of P.Pastoris by Dot blot and DNA sequencing. Pastotis進行整合,經G418篩選得到25個高拷貝轉化子,經DNA斑點試驗和DNA測序證明外源基因E2穩定地整合到P.;Pastoris染色體中。
- The methods include using Agr obacterium-mediated transformation, electroporation, gene gun bombardme nt and polyethyleneglycol (PEG)-mediated direct DNA transfer. 果樹基因轉化方法主要包括農桿菌介導法、電激法、基因槍法、PEG轉化法等。
- The mutations were detected by direct DNA sequencing. Results All samples examined carried mtDNA A1555G mutation in 12SrRNA gene and G7444A mutation in COI/tRNASer(UCN) gene. 結果測序結果表明,此家系線粒體DNA12SrRNA基因中存在著A1555G突變,COI/tRNASer(UCN)基因中存在著G7444A突變。
- A deletion mutation with frameshift of neurofibromatosis type 2 gene was detected by DNA sequencing. 以DNA序列分析出,在第二型纖維細胞瘤基因上發生染色體缺失造成移碼突變。
- Authentication,PCR and DNA sequencing showed the recombinant plasmid of human MIA/CD-RAP was successfully constructed. 酶切電泳和DNA測序結果表明,成功地克隆了人黑素瘤M IA/CD-RAP cDNA。
- B7 1(CD80) cDNA was cloned by RT PCR from human B lymphma Raji cell line and confirmed by DNA sequencing. 應用RT-PCR技術從人B淋巴瘤細胞系Raji中克隆到B7-1(CD80)cDNA,並經測序證實。
- Methods STK 11 germline mutation was analysed by DNA sequencing in 18 unrelation patients with PJS. 方法 用 DNA直接測序方法 ,對 18個家系的 PJ綜合征患者 STK1 1 基因 9個外顯子進行研究。
- The more varied the organisms, the more varied the DNA sequences. 生物變化越多,DNA序列的變化也就越多。
- Methods:PCR,denatur ing gradient gel electrophoresis (DGGE) atd DNA sequencing were used. 方法:採用多聚酶鏈反應(PCR),變性梯度凝膠電泳(DGGE)和DNA測序等方法對上海地區血友病甲基因突變進行檢測。
- The more varied the organisms,the more varied the DNA sequences. 生物變化越多,DNA序列的變化也就越多。
- DNA microarray has been applied to DNA sequencing,pharmaceuticals analysis,gene expression and so on. DNA微集陣列已廣泛用於DNA測序、藥物分析、基因表達等研究領域。