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- The Tat signal peptide detecting vector of PGA were constructed. 構建了Tat信號肽檢測載體。
- We add the 5'AOX1 primer and 3'AOX1 primer amplify pPIC9K and alpha-factor signal peptide sequence was obtained. 考慮到克隆方便,在序列5』端和3』端分別引入BamHI和EcoRI 位點,並在兩個酶切位點的外側分別加上保護鹼基。
- Once across the membrane, the signal peptide is cleaved by the signal peptidase and the process protein is released. 信號肽是疏水的,與膜上結合的蛋白接近,拉著後面的親水多肽跨膜,一旦跨過膜,即被信號肽酶切除,釋放出加工的蛋白。
- The location of the signal peptide of this peptide chain is 1-26, the breaking point is between 26 and 27 (GAA-AF). 應用神經網路演算法和隱馬氏模型分析結果顯示該肽鏈信號肽位置為1-26,斷裂點位置是26-27(GAA-AF)。
- The extramembrane region consists of 758 aminoacids, including signal peptide containing 31 amino acids at N-terminus and seven Ig-like domains. 胞外區由758個氨基酸組成,包括N端31個氨基酸的信號肽和七個類似免疫球蛋白(Ig)的結構域。
- There were no signal peptide and N-glycosylation sites, while had 4 conservative Cys amino acid in the deduced amino acid sequence. 在推導的貓IL-18氨基酸序列中,無信號肽序列和潛在的N-聯糖基化位點,但存在4個Cys殘基。
- The signal peptide at the N- termination can lead the protein to the periplasm.Six histidines at the C- termination will let the purification easy. 在質粒pET-20b(+)中,重組的蛋白會在N-末端帶有一個信號肽序列,它可以使重組蛋白分泌到細胞的周質空間處,而其在C-末端還有帶有6個組氨酸的標籤,這樣就使得重組蛋白的分離純化更加的方便,簡單。
- The artificial signals can then be processed and sent through the optic nerve to the brain. 然後,人造的信號經處理,通過光神經送給大腦。
- Proteins secreted by one step into the periplasm or through Bayer's junctions to the extracellular environment have a signal peptide. 蛋白可直接分泌到周質中,或通過Bayer接點分泌到胞外環境中的蛋白有一個信號肽。
- In this paper, signal peptide was added before the N-terminus of CEMA with the intention to reduce its toxic to plant cells while maintaining the strong antibacterial activity. 本文通過在CEMA的N端添加信號肽,對其進行改造,以探索不降低抑菌作用的同時,降低其對植物細胞毒害作用的可能性。
- GliTd-3 and GliTd-4 were both 861bp, and their deduced amino acid sequences were both 267 residues with a 19-residue putative signal peptide (absent from mature proteins). 而GliTd-1和GliTd-2編碼區分別長821bp和859bp,由於編碼區內存在提前終止密碼子和插入/缺失引起的移碼突變,推測其為不表達的假基因。
- With the signal peptide analysis software of the TMHMM2.0(http://genome.cbs.dtu.dk/services /TMHMM-2.0), the preceding peptide of Vip3A-WB5 was a transmembrane peptide. 通過TMHMM2.;0(http://genome
- Fibroblast growth factor(FGF-1)lacks amino-terminal signal peptide, so it can』t release through the classical endoplasmic reticulum(ER)-Golgi pathway. 酸性成纖維生長因子-1(FGF-1)由於氨基端缺乏信號肽序列,不能通過經典的內質網-高爾基體途徑釋放。
- With common molecular biology technique,we cloned SCF gene including signal peptide coding sequence coded signal peptide and constructed its expression plasmid. 採用常規的分子生物學技術,從小鼠骨髓細胞中克隆了含信號肽序列的SCF,並構建了表達載體。
- This article reviews signal peptide sequence about its structural feature,the common used signal peptide and the construction of vector with high secretory. 本文將從信號肽結構特徵、常用的信號肽序列以及構建高分泌型載體等方面進行綜述。
- Signal peptide was found on former 20 amino acids of both two coding regions of protein, one strong and one feeble transmembrane helices were also found. 2. 並在兩種酶蛋白編碼區的前20個氨基酸發現信號肽序列,通過跨膜區預測,均發現一個較強的跨膜信號和一個較弱的跨膜信號。
- The fusion DNA fragment of the lactococcal extracellular protease USP45 signal peptide, passenger protein and SBC were expressed in Lactococcus lactis and Lactobacillus paracasei. 融合乳酸菌胞外蛋白酶USP45訊息胜肽、乘載蛋白及表層蛋白質靜默基因C端123個胺基酸,表現於副乾酪乳桿菌或乳酸鏈球菌。
- This dress is made of artificial fibers. 這件衣服是人造纖維的。
- Her artificial gaiety disguised an inner sadness. 她以矯揉造作的快樂掩飾著內心的悲痛。
- Obtaining of SPCEMA(signal peptide modified CEMA)SPCEMA(187bp) was amplified with two long complementary primers (P2 and P3) and two primers (Pl and P4) containing restriction enzyme recognition site. 以兩條部分重疊長鏈引物P_2和P_3延伸產物為模板,P_1和P_4為正、反引物進行PCR擴增,獲得了改造的抗菌肽基因SPCEMA(187bp)。