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- Gelfiltration negative anion exchange column chromatography with high performance liguid chromatography system and plant hemagglutinin affinity chromatography were used for the isolation and purification of blood group substances from saliva. 方法:應用凝膠過濾、陰離子高速液相色譜及PHA親合層析法,從人唾液中分離純化血型物質。
- Galangin was obtained by on-step column chromatography of polyamide only once. 運用聚醯胺柱層析-次分離,獲得有效成分高良姜素。
- Its homogeneity was identified with Sepharose 6B column chromatography. 紫外光譜顯示它不含蛋白質、多肽及核酸。
- Plasma membrane proteins of sperm cells from Zea mays were passed through affinity column, separated by SDS|PAGE and stained using Con A|HRP. 用親和層析,SDS?PAGE 及印跡技術,從玉米精細胞中分離純化質膜蛋白。
- In this paper, we extracted the antioxidative substance from Alpinia oxyphylla Miquel by using column chromatography. 摘要探討了干柱層析法分離益智渣及益智乙醇提取物的抗氧化成分。
- METHODS: Alcoholic extraction, silica gel, ODS column chromatography and recrystallization were used. 方法:採用工業酒精提取、膠柱層析、DS柱層析和重結晶等方法。
- MethodsMonoclonal antibody is an IgG1 antibody, sepharose 4B and SPA affinity column were applied for purifi cation of AchE monoclonal antibody (3F3), using papain cut 3F3,to get Fab fragme nt and immune mice with Fab. 方法 AchE單克隆抗體是一種IgG1抗體 ,先用木瓜蛋白酶酶切 ,然後採用AchE Sepharose 4B和SPA親和層析柱進行提取純化 ,獲得純化的單克隆抗體Fab段。
- METHODS CTX-d was fractionated and purified from Naja naja atra venom by column chromatography and RP-HPLC. 方法應用柱層析及RP-HPLC,從眼鏡蛇毒粗毒中分離純化細胞素素(CTX)。
- Results Galangin was obtained by on-step column chromatography of polyamide only once. 結果運用聚醯胺柱層析-次分離,獲得有效成分高良姜素。
- Objective To separate flavonol and flavanonol by the column chromatography of polyamide. 目的採用聚醯胺柱色譜分離黃酮醇與二氫黃酮醇類化合物。
- To set up a sephadex column chromatography method for the determination of polymer impurity in cefotetan disodium. 目的建立葡聚糖凝膠色譜法測定頭孢替坦二鈉中聚合物的方法。
- Results Pure flavonol and flavanonol were obtained by the column chromatography of polyamide. 結果運用聚醯胺柱色譜法一次分離得高純度黃酮醇與二氫黃酮醇。
- The molecular weight of the antibiotic substance is 48000 Dalton,tested by sephadex-G75 gel column chromatography. 而且用Sephadex-G75凝膠柱層析法測得抗生物質的分子量為48000道爾頓
- Conclusion: Silica gel column chromatography and crystallization are simple and rapid methods to isolate gardenoside. 結論:柱層析和重結晶方法是簡單、速分離梔子苷的有效辦法。
- The potential of sample clean-up with sol-gel affinity columns is demonstrated giving applications in environmental, food and clinical analysis. 以這些親生物性溶膠管柱來作樣品清理的應用潛力,已由環境、食物及醫療上獲得證明。
- The extract was further purified by column chromatography to get the acicular crystals of reserpine of 99. 將萃取所得產物用硅膠柱色譜進一步分離提純;得到了質量分數為99.;8%25的利血平針狀結晶。
- Methods The compounds were isolated by silica gel column chromatography and identified by spec-tral analysis. 方法通過硅膠柱色譜法分離純化化合物,譜學方法鑒定其結構。
- Then the extracted material was sequently filtered and sperated by means of silica gel column chromatography. 提取物通過硅膠柱層析分離,得到無辣味的紅色色素。
- Silica gel column chromatography was used to separate and purify total alkaloids from Fritillaria ussuriensis Maxim. 目的:對東北特產平貝母的生物鹼進行分離純化研究。
- Results 98% purity was reached through 14F7 monoclonal antibody column, and 95% purity was gained after goat anti human Fab fragment column. But yield ratio of the two affinity columns was low, being 35% and 55%, respectively. 結果3種純化方法中,14F7單克隆抗體柱純化的Fab抗體的純度達98%25左右,Fab抗體柱純化的Fab抗體的純度為95%25,但這兩種親和柱的目的蛋白收率都不高,分別為35%25、55%25。