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- After transfection of pPTA1/Ig into COS 7 cells by DEAE dextran, expression of a secreting fusion protein was identified by affinity chromatography and sandwich ELISA assay with anti PTA1 mAb and HRP anti hIgFc mAb. 重組載體通過DEAE?dextran法轉染COS?7細胞,經夾心ELISA及親和層析、SDS?PAGE鑒定融合蛋白的表達及免疫學活性。
- DEAE dextran 二乙氨(基)乙基葡聚糖
- Pinocytosis of dextran was unaffected. 對葡聚糖的胞飲作用無影響。
- After purification with DEAE Sepharose F. 結果:成功得到海螵蛸粗多糖。
- Dextran sulfate sodium salt from Leuconostoc spp. 硫酸葡聚糖。
- After ATF gene transfection, the ATF mRNA was obviously expressed. 結果表明,轉染ATF基因后,腫瘤細胞呈現ATF基因的明顯表達;
- Transfection, make stable C/EBP p expression cell line. 質粒轉染,製備穩定表達C尼BPp的細胞系。
- Cell density was associated with PEI/DNA transfection efficiency. 細胞密度影響轉染效率;
- Gene transfection is the key technique of gene therapy. 摘要基因治療的關鍵技術是基因轉移。
- Ingredient:Plant coenzyme Q10, Extended protein factor, dextran. 成份:植物輔酶Q10,伸展蛋白因子,葡聚糖。
- Methods: The polysaccharide was got by the extraction, DEAE chromatography etc. 方法:依次用水提取、過濾、去蛋白、DEAE纖維素層析、冷凍乾燥獲得大蒜多糖;
- Objective To construct Giardia canis virus(GCV) transfection vector. 目的構建犬賈第蟲病毒(Giardia canis virus,GCV)轉染載體。
- Methods Clinical study was made in 13 cases with ARF by low Molecular Dextran. 方法:回顧性分析13例低分子右旋糖酐致急性腎功能衰竭的臨床資料。
- Iron Dextran manufactured by Herbon is the best iron product in the world. 漢邦公司生產的右旋糖酐鐵是世界上最好的用於防治動物及人體貧血的藥物之一。
- GFP can be used as a reporter system for optimizing transfection of K562 cells. 結論:電轉染較脂質體轉染K562細胞的效率高,GFP可作為報告系統優化K562細胞轉染條件。
- The inhibitory effect of gene transfection on solid tumor was observed in mice. 小鼠實體瘤塊模型研究基因轉染抑制肝癌生長作用。
- Hoechst and HE staining showed that apoptosis was induced after transfection. 細胞HE染色部分細胞呈典型的凋亡細胞形態學改變;
- The concentration of dextran solution was measured by testing its turbidity value using ethanol as the precipitant. 以乙醇為沉澱劑;測定水溶液中葡聚糖溶液濁度的方法測定葡聚糖的濃度.
- For DEAE Sepharose FF weak anion-exchange resin chromatography, the optimum operation was with buffer Tris-HCl(Ccl_ =20mM),pH8.0 and the concentration of NaCl was also 0.4M. 對於DEAE弱陰離子交換層析柱,採用Tris-HCl(Ccl_ =20mM),pH8.;0緩衝體系;同時洗脫緩衝體系也維持NaCl濃度為0
- Comparison of homogeneous HDL-C assay with dextran sulfate 50- magnesium chlorideprecipitation method. 高密度脂蛋白膽固醇直接測定法與硫酸葡聚糖50-鎂法比較。