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- GHR基因5』端Promoter區growth hormone receptor(GHR)gene 5' promoter region
- 擴增p 16 promoter一luc一pGLZ一Basic報告基因表達質粒並提取其DNA。P16-promoter-luc-pGL2-Basic plasmid DNA was prepared after plasmid amplification, and digested by EcoR I and Hind III.
- F-actinF-actin
- β-actinβ-actin
- actin基因actin gene
- α-SM-actinα - smooth muscle - actin
- U3R調控區與pEGFP-N載體融合構建了雞CES細胞特異表達載體pEGFP-cENS2-Promoter,通過轉染胚盤細胞、CEF細胞驗證其特異的啟動子活性。The CMV promoter was cut off from pEGFP-N1, and the putative U3-R fragment was inserted just upstream of GFP gene. Results of restriction digestion and DNA sequencing showed that the vector pEGFP-cENS2-Promoter for expressing in CES cell was successfully constructed.
- actin結合活性actin binding activity
- 為此，本文構建了植物表達載體pZPZ 12一rd29A promoter一ABPg和pZP212一rd29A promoter一ABPg一GFP，並成功獲得了轉基因的擬南芥植株。To analysis the expression of ABP9 in transgenic plant, the plant expression vectors pZP212-rd29Apromoter-ABP9 and pZP212-rd29Apromoter-ABP9-GFP was constructedBy means of Agrobacterium tumefaciens -mediated, two constructs were transformed into plant then obtained transgenic plant.
- β-actin基因β-actin gene
- -βactin啟動子β-actin promoter
- 接著,用Promoter Proscan Version 1.7軟體進行基礎啟動子分析,發現兩段啟動子序列的基礎啟動子區均在在MOC1基因起始密碼子ATG前639bp-389bp之間。We analysised the core promoter region with software of Promoter Proscan Version 1.7, the result indicated that the two fragment have core promoter region in 639bp to 389bp before initial code ATG of M0C1 gene.
- 雞β-actin啟動子Chicken β -actin promoter
- EMA、Actin、S-100、ER陰性;Negatived for EMA,Actin,S-100,ER;
- 標記F-actin,熒光顯微鏡下觀察。F-actin was labled and examined using fluorescence microscope.
- 高糖組F-actin熒光強度為對照組的44.5%；High glucose can decrease the fluorescent intensity of F-actm to 44.5%25 (p<0.01);
- MOMA-2表達增加,SM-actin表達降低(P<0.05)。Even,upregulation of MOMA-2 and downregulation of SM-actin were also detected in latter(P<0.05).
- 高速電視攝影管
- 電離輻射對血管內皮細胞骨架蛋白F-actin影響的激光共聚焦顯微鏡觀察The Observation of the Influence of Ionizing Radiation on the Image of F-actin in Vascular Endothelial Cells with Confocal Laser Scanning Microscope
- 結果表明,LIM結構域2在hhLIM與actin相互作用及調節actin細胞骨架組構中起決定性作用.In conclusion, LIM domain 2 at the C-terminus of hhLIM plays a central role in F-actin polymerization and cytoskeleton stabilization, whereas the first LIM domain is essential for the nuclear localization of hhLIM.
