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- The concentration ofcalcium was assessed by ACAS (adherent cell analysis and sortingcytorneter). 用粘附式細胞儀測定細胞內鈣離子濃度;
- The sample of single, live, adherent cell was studied by the confocal microscopy. 激光掃描共聚焦顯微鏡是以單個的、活性的、貼壁的細胞標本為主要的研究對象。
- The adherent cells were gained by cultivated in vitro. 體外培養以獲得貼壁細胞。
- Applicable to primary cells (HUVEC, primary Neurons, etc.) And other adherent cell lines. 用於原代細胞(人臍靜脈內皮細胞,原代神經細胞等)和其他貼壁細胞系。
- Culture of Primary Adherent Cells and a Continuously Growing Nonadherent Cell Line Cheryl D. 介紹細胞培養的基礎知識。英文教材,PDF文件。
- Effect of procainamide (Pro 0. 5mmol/L) on single human platelet cytosolic free Ca2+ induced by thrombin was investigated by ACAS-570 (Adherent cell analysis and sorting cytometer). Fluo-3-loaded platelets were immobilized on a fibrinogen-coated surface. 用570型粘附式細胞儀動態觀察普魯卡因胺(Procainamide,Pro)對凝血酶激活的人單個血小板細胞內遊離Ca2+的影響。
- By using fluorescence staining method, the effect of receptor-mediated endocytosis on intracellular pH (pHi) of mouse macrophages was studied with adherent cell analysis and sorting cytometer 570 (ACAS 570). 用pH值敏感熒光探針Snaflcalcein-AM標記體外培養48小時的小鼠腹腔巨噬細胞,570型粘附式細胞儀觀察巨噬細胞在對刀豆素A進行受體介導內吞過程中細胞內pH值的變化。
- The experiment revealed that in order to induce antibody in vitro, the adherent cells were necessary. 此實驗還證明體外誘生抗體必須有粘附細胞參與。
- The suppressive activity of non-adherent cells (T cells) and adherent cells could be detected, too. 將去除了巨噬細胞的帶瘤小鼠脾細胞進一步經尼龍毛分離,發現尼龍毛粘附細胞及非粘附細胞均產生一定的抑制作用。
- There are 8.825 + 2.958 adherent cells per 100 peripheral blood mononuclear cells. 每100個外周血單個核細胞(PBMC)可獲得8.;825±2
- Cord blood adherent cells display fibroblast-like or spindle shape,and their reproductive cycle: G_0/G_1was 85.12%, the doubling time was 48 hours. 臍血貼壁細胞呈長梭形、針形或三角形;增殖周期G0/G1為85.;12%25;倍增時間為48h。
- After culture them for 14 days, collect adherent cells ,detect CD86,CD83, CD14, HLA-DR and other immune factors with flow cytometer. 14d后,收集貼壁DC細胞,分別用流式細胞技術檢測CD83、CD86、CD14、HLA-DR等免疫表型。
- Methods The umbilical cord blood CD34 + cells were cultured in Dexter system in order to observe the biological behavior of adherent cells in vitro. 方法 分離人臍血CD34+ 細胞 ,採用Dexter法培養 ,對貼壁細胞進行觀察和鑒定。
- Objective To study the growth characteristics of cord blood adherent cells and provide the hematopoietic microenvironment. 目的觀察臍血貼壁細胞生長特性及提高體外培養臍血貼壁細胞成功率,為造血幹細胞培養提供基質環境。
- Adherent cell analysis and sorting cytometer, ACAS 本實驗應用粘附細胞儀
- The percentage of P210 positive cells was significantly lower in non adherent cells after 10 days of culture in Dexter system, comparing with that in non cultured BMMNC. BMMNC經培養后非貼壁細胞中P2 10陽性細胞比例明顯減少 ,以含DC者最為顯著 ,含rhIL 2者次之。
- Objective: To establish a method for isolation and purification of fetal membrane derived adherent cells (FMDACs) , and investigate their biological characteristics. 目的:建立體外分離純化胎膜組織貼壁細胞(fetal membrane derived adherent cells, FMDACs)的方法,並且研究FMDACs的基本生物學特性。
- Immunocytochemistry and flow cytometry analysis showed that almost all of the adherent cells were vWF positive and around 90% were able to bind UEA I specifically. 免疫細胞化學和流式細胞術的結果顯示 ,幾乎所有貼壁細胞為vWF陽性 ,且約 90%25的細胞具有UEA I結合能力。
- The adherent cells and non-adherent cells were collected respectively,which were identified by light microscope,electron microscope, immunochemistry and flow cytometric analysis. 用光鏡、電鏡、細胞免疫化學、流式細胞儀法鑒定該兩種細胞類型。
- GU XF, MA BY, WU Y, et al.Changes in the composition of adherent cells in long-term human bone marrow culture[J].ATCA Academiae medicinae shanghai, 1994, 21(5):369-372. [3]顧小鋒;馬伴吟;吳口;等.;人骨髓細胞體外長期培養基質細胞組成及其變化[J]