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- PCR requires the synthesis of oligonucleotide primers complementary to the target DNA. The entire sequence of the target DNA does not need to be known, only the flanking regions. 就整個PCR擴增技術而言,引物的設計佔有十分重要的地位,它的優劣直接關係到PCR的特異性與成功與否。
- Based on the study, it is very convenient for making a deep research of the foreign gene expression under the regulation of CSN2 flanking region. 為進一步利用CSN2質粒兩端的調控序列, 插入新的基因, 研究其表達功能, 或者進行乳腺生物反應器的研究奠定了基礎。
- Methods: 201 type 2 diabetics, 4 idiopathic type 1 diabetics and 100 control subjects were included in the study.Two exons of IPF-1(including the coding and flanking regions) were amplified using PCR. 方法:選擇2型糖尿病201例、特發性1型糖尿病4例及正常對照組100例作為研究對象,對IPF-1基因的兩個外顯子序列(包括編碼區及側翼區)進行PCR擴增,PCR產物經純化后,經ABI 377 DNA 全自動測序儀對所有PCR產物直接測序。
- Cloning and sequence analysis of 5'flanking region of prolactin gene in Small Tail Han sheep 小尾寒羊催乳素基因5'側翼調控區的克隆與序列分析
- Positional identification of transcription regulation elements in 5'flanking region of NGAL gene NGAL基因5'側翼區轉錄調控元件的分段定位鑒定
- 5 'flanking region 5』側翼序列
- These fish vary in weight from 3 lb to 5 lb. 這些魚的重量從3磅到5磅不等。
- The going rate for freelance work is 5 an hour. 自由職業者的現行酬金是每小時5英鎊。
- 5' flanking region 5』側翼區
- Many children under 5 go to nursery school. 5歲以下的許多兒童都上幼兒園。
- Meals average out at 5 per head. 膳食平均每人5英鎊。
- Children under 5 can travel for nothing. 5歲以下兒童可免付旅費。
- Comparative analysis of genetic variations of pgm locus and its flanking regions in Yersinia pestis 鼠疫耶爾森菌pgm位點及其側翼序列遺傳變異的比較分析
- Even the cheapest was priced at 5. 即使最便宜的也標價5英鎊。
- The liquid was 5 litres in volume. 該液體的體積為5升。
- The ratios of 1 to 5 and 20 to 100 are the same. 1與5之比和20與100之比相同。
- The winning time was 1 minute 5 seconds. 獲勝的時間是1分零5秒。
- I found a 5 note on the pavement. 我在人行道上撿到了一張5英鎊的紙幣。
- He is 5 centimeters taller than her. 他比她高5公分。
- He is 5 centimetres taller than her. 他比她高5公分。