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- 地高辛標記引物酶顯色法檢測HBV基因多態性及應用Application of enzyme coloration with digoxin-labeled primers for detection of HBV gene polymorphism
- 方法 應用聚合酶鏈反應(PCR)方法,採用熒游標記引物和377型DNA序列自動分析儀,分析21例 GBM 9號染色體上 20個微衛星多態性標記的LOH。Methods 20 loci on chromosome 9 were examined to detect loss of heterozygosity(LOH) in 21 cases of glioblastoma by PCR based microsatellite polymorphism analy-ses, in which fluorescence-labeled primers and Perkin Elmer 377 DNA Sequencer were applied.
- 熒游標記引物複合擴增短串聯重複序列技術用於異基因造血幹細胞移植植活測定A study on technique of multiplex polymerase chain reaction with fluorescence labeling primers for short tandem repeats to document engraftment following allogeneic stem cell transplantation and its application
- 任意引物PCRarbitrarily primed PCR
- 標記mark
- 二引物PCRTP-PCR
- 雙引物PCRDouble - primer PCR
- 方法 應用四色熒游標記引物複合擴增技術 ,對鄂倫春族 10 1名無關個體的血樣 15個STR基因座進行分型研究 ,計算各基因座的相關群體遺傳學參數。Method DNA samples from 101 unrelated individuals in Chinese Olunchun population were screened by Power Plex 16 System and ABI 3100 Genetic Analyzer.
- 引物primer
- 隨機引物PCRarbitrarily primed PCR(APPCR)
- 組合引物PCR法grouping primers PCR
- 半隨機引物PCRSemi-random primer PCR
- 方法應用聚合酶鏈反應(PCR)方法,通過熒游標記引物和377型DNA序列自動分析儀,對20例原發性膠質母細胞瘤患者7號染色體上的22個微衛星多態性標記進行雜合性丟失(lossofheterozygosity,LOH)分析。Methods All of the 22 microsatellite polymorphism markers on chromosome 7 in 20 cases with GBM were detected by polymerase chain reaction (PCR) technique with fluorescence-labeled primers and Perkin Elmer 377 DNA sequencer and the loss of heterozygosity (LOH) in them was analyzed.
- 引物原位標記primed in situlabelling; RINS
- 型特異性引物PCRType-specific primer PCR
- 熒游標記引物fluorescent dye primer; fluorescently-labeled primer
- 隨機引物法標記Random Prime Labeling of DNA
- RNA任意引物PCRRNA arbitrarily primed PCR
- 隨機引物標記法random primering
- 因而,設計PCR引物時宜選擇Takahashi等分離株為標準。In China these viruses share high homology with Takahaski』s strains which might be good standards for primer designation.