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- Objective To investigate the effect of bcr abl fusion gene on CML cell apoptosis. 目的研究bcr?abl融合基因在慢性粒细胞白血病细胞(CML)凋亡调控中的作用。
- The results showed that better reaction conditions were gained by exploration of hybridizotion temperature and elution conditions, bcr abl fusion gene in leukemia cells was detected by prepared miccroarray. 结果表明 :通过对不同杂交温度、洗涤条件等的探索获得了较好的反应条件并用制备的芯片检测出了细胞株中的bcr abl融合基因。
- Method:The fragement of Bcr abl fusion gene was amplified from K562 cells of CML by RT PCR and was cloned into the downstream of GST gene in pGEX 6P 1 vector according to the current open reading frame. 方法 :以慢性粒细胞白血病 (CML)K5 6 2细胞株总RNA作为模板 ,采用RT PCR方法扩增包含Bcr abl(b3a2 )融合位点周围的基因片段。
- Induction of CTL by immunization of bcr abl fusion gene bcr-abl基因免疫诱导特异性细胞毒性T淋巴细胞杀伤功能
- Inhibition of apoptosis by bcr abl fusion gene in K562 cells bcr-abl融合基因抑制K562细胞凋亡的研究
- EXPERIMENTAL STUDY ON THE DETECTION OF BCR ABL FUSION GENE IN LEUKEMIAS BY RT PCR 白血病 BCR-ABL 融合基因逆转录 PCR 检测中的实验因素探讨
- Growth of K562 cells and the expression of CyclinD1 gene and bcr abl fusion gene effected by Genistein Genistein对K562细胞生长及CyclinD1基因和bcr-abl融合基因表达的影响
- Study on the in vitro cleavage abilities of ribozymes specific to different sites of bcr abl fusion gene and their induction of apoptosis in K562 cells Bcr-abl特异性系列核酶体外切割活性比较及诱导K562细胞凋亡的实验研究
- bcr abl fusion gene formation due to chromosome translocation may be the major mechanism of CML via inhibition of apoptosis. bcr?abl融合基因可抑制CML细胞凋亡,这可能是CML发病的主要机制。
- To explore the application value of bcr abl fusion gene deterction microarray in diagnosis, typing, choosing of treatment variant and prognosis judgment, probe for fusion gene detection was designed, oligonucleotide microarray was prepared; 为了研究bcr abl融合基因检测芯片在白血病诊断、分型、治疗方案选择及预后判断中的应用价值 ,设计了融合基因检测探针 ,制成寡核苷酸芯片 ;
- A rapid reverse transcriptase transcribed PCR(RT PCR) was established to detect different types (b3a2, b2a2, B1a2) of bcr abl fusion mRNAs in leukemias. 建立白血病中费城(Ph)染色体上独特的bcr-abl融合基因的逆转录PCR(RT-PCR)快速检测方法。
- Secondary, a 376bp fragment containing the bcr abl fusion site was amplified by PCR. This fragment was cloned into pBluescript KS to be used as the template vector for in vitro cleavage experiment. 此外, 通过PCR 方法, 扩增了bcr?abl 融合位点附近约376bp 的序列,成功地构建了bcr?abl 融合基因体外转录载体。
- LDH assay showed CTL was induced by the bcr abl immunization. 4bcr- abl基因免疫后的小鼠能有效诱导出特异性细胞毒 T细胞 (cytotoxic T lymph-cyte;CTL) .
- Firstly, three hammer head ribozymes specific to the fusion point of bcr abl were designed and cloned into a modified in vitro transcription vector pDES. 为此,我们针对bcr?abl 融合位点设计、合成了3 个相邻的锤头状核酶。
- Expression of fusion gene in transfected COS 7 cells was justified. 重组质粒转染COS 7细胞后经检测证实 ;该融合基因能在真核细胞中表达 .
- Apoptosis of K562 cells was significantly increased associated with inhibition of bcr abl expression. bcr?abl融合基因表达受抑后,K562细胞凋亡显著增加。
- Mimics of both types of BCR ABL cDNA were achieved and the validity was verified with restriction endonuclease. 经酶切分析证明 ,两型BCR ABLmRNA均可通过此方法得到相应的cDNA参照物。
- The PTK activity of BCR ABL protein was also mildly decreased in CML monouclear cells at 60 h. As2 O3作用 6 0h ,可使CML患者骨髓单个核细胞BCR ABL蛋白PTK活性轻度下降。
- Uncommon bcr/abl fusion gene may occur in typical Ph(+) CML patient. 少见型bcr/abl融合基因可见于典型Ph染色体阳性CML患者并产生异常的RT PCR扩增带。
- The transfected cells expressed IL-18 fusion gene and 18 kD IL-18 protein. IL-18转染细胞表达IL-18融合基因及18 kD蛋白。
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