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- In order that bar gene efficiently transcript and translate in eukaryote, the sequence next to ATG was modified by PCR. 为了目的基因在真核生物中有效的翻译和表达,通过PCR方法对bar基因起始密码子ATG旁侧序列进行改造。
- The integration of the bar gene in the genome of the transgenic rice plants was shown by PCR and Southern analysis. 经PCR及Southern分析,在转基因植株基因组中均检测到了bar基因的整合;
- The transgenic rice carring Bar gene was identified to high tolarance to the herbicide Basta containing PPT active elements. 转Bar基因水稻对以PPT为活性成分的Basta除草剂有明显抗性。
- Specific protein band expressed by bar gene could also be utilized to identify the transgenic plants from non-transgenic plants. 此外,不同水稻品种蛋白带的差异也可用于区分转基因与非转基因的水稻品种。
- This protein can be used for foodsafety assessment detection of genetically modified products with bar gene and for production of anti-bar antibody for ELISA detection. 该蛋白可以替代植物外源蛋白进行转bar基因产品的食用安全性检测,也可用于转bar基因产品ELISA检测的抗体制备。
- There were 27 resistant plants in the 42 regenerated plantlets By PPT (150mg/L )screening. This result indicated bar gene was integrated and expressed in the wheat genome. 对所得T0代42株再生苗的叶片进行PPT涂沫抗性测定(150mg/L),结果有27株表现抗性,说明Bar也整合到小麦基因组中。
- For gene bombardment, somatic embryo calli of "Zhong Mu No.l" were taken. The E. coli plasmid obtained through the generosity of Cornell University which contained lea3 gene banded to bar gene was used. 用以lea3基因为目的基因,bar基因为选择基因的质粒pBY520包裹微弹,基因枪轰击苜蓿胚性愈伤组织。
- Bar-transgenic wheat, Nong4- Bar gene transferred into D genome with the herbicide-resistant function, can be chosen as the parents to breed the varieties which can resist herbicide by the traditional way. Nong4是Bar基因插入在受体小麦D染色体组上的转Bar基因小麦,由于其具有抗除草剂功能,转Bar基因小麦将成为选育高产优质抗除草剂小麦品种的重要材料。
- Based on the results of optimization, the Anti-Trxs gene and Bar gene were co-transferred into the immature embryos(cali) from Yumai18-64 as receptors and 42 resistant plantlets of T0 generation were obtained from 2110 transferred embryos. 再生体系优化的基础上,以筛选出的优良受体豫麦18-64为受体材料,用基因枪法进行了目的基因(Anti-TrxS)和标记基因(Bar)的共转化和再生培养,从接种的2110个幼胚中,获得T0再生植株42株。
- Barring accidents, I'll be there. 除非有意外事故,我会一定到场的。
- I want to sing at a karaoke bar next. 接下来,我想去卡拉OK酒吧唱歌。
- Barring accidents, we should arrive on time. 倘无意外,我们应准时到达。
- He will have a drink in the bar after work. 下班之后他总去酒吧喝一杯。
- Poor health may be a bar to success in life. 健康不佳可能成为人一生中取得成功的障碍。
- She's go to play the opening bar of the sonata. 她准备演奏这支奏鸣曲的开始数小节。
- The bar in the slum is a den of iniquity. 这个贫民窟里的小酒吧是一个充满罪恶的场所。
- The ship crossed the bar safely. 船平安地穿过沙滩。
- The drunk man lost his pouch in the bar. 那个喝醉了的人在酒吧里丢失了钱袋。
- The whole class is here bar two that are ill. 除两人生病外,全班都到齐了。
- Studies and Utilization of Bar Gene and Its Transgenic Rice Bar基因及转Bar基因水稻的研究和利用