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- The Tat signal peptide detecting vector of PGA were constructed. 构建了Tat信号肽检测载体。
- We add the 5'AOX1 primer and 3'AOX1 primer amplify pPIC9K and alpha-factor signal peptide sequence was obtained. 考虑到克隆方便,在序列5’端和3’端分别引入BamHI和EcoRI 位点,并在两个酶切位点的外侧分别加上保护碱基。
- Once across the membrane, the signal peptide is cleaved by the signal peptidase and the process protein is released. 信号肽是疏水的,与膜上结合的蛋白接近,拉着后面的亲水多肽跨膜,一旦跨过膜,即被信号肽酶切除,释放出加工的蛋白。
- The location of the signal peptide of this peptide chain is 1-26, the breaking point is between 26 and 27 (GAA-AF). 应用神经网络算法和隐马氏模型分析结果显示该肽链信号肽位置为1-26,断裂点位置是26-27(GAA-AF)。
- The extramembrane region consists of 758 aminoacids, including signal peptide containing 31 amino acids at N-terminus and seven Ig-like domains. 胞外区由758个氨基酸组成,包括N端31个氨基酸的信号肽和七个类似免疫球蛋白(Ig)的结构域。
- There were no signal peptide and N-glycosylation sites, while had 4 conservative Cys amino acid in the deduced amino acid sequence. 在推导的猫IL-18氨基酸序列中,无信号肽序列和潜在的N-联糖基化位点,但存在4个Cys残基。
- The signal peptide at the N- termination can lead the protein to the periplasm.Six histidines at the C- termination will let the purification easy. 在质粒pET-20b(+)中,重组的蛋白会在N-末端带有一个信号肽序列,它可以使重组蛋白分泌到细胞的周质空间处,而其在C-末端还有带有6个组氨酸的标签,这样就使得重组蛋白的分离纯化更加的方便,简单。
- The artificial signals can then be processed and sent through the optic nerve to the brain. 然后,人造的信号经处理,通过光神经送给大脑。
- Proteins secreted by one step into the periplasm or through Bayer's junctions to the extracellular environment have a signal peptide. 蛋白可直接分泌到周质中,或通过Bayer接点分泌到胞外环境中的蛋白有一个信号肽。
- In this paper, signal peptide was added before the N-terminus of CEMA with the intention to reduce its toxic to plant cells while maintaining the strong antibacterial activity. 本文通过在CEMA的N端添加信号肽,对其进行改造,以探索不降低抑菌作用的同时,降低其对植物细胞毒害作用的可能性。
- GliTd-3 and GliTd-4 were both 861bp, and their deduced amino acid sequences were both 267 residues with a 19-residue putative signal peptide (absent from mature proteins). 而GliTd-1和GliTd-2编码区分别长821bp和859bp,由于编码区内存在提前终止密码子和插入/缺失引起的移码突变,推测其为不表达的假基因。
- With the signal peptide analysis software of the TMHMM2.0(http://genome.cbs.dtu.dk/services /TMHMM-2.0), the preceding peptide of Vip3A-WB5 was a transmembrane peptide. 通过TMHMM2.;0(http://genome
- Fibroblast growth factor(FGF-1)lacks amino-terminal signal peptide, so it can’t release through the classical endoplasmic reticulum(ER)-Golgi pathway. 酸性成纤维生长因子-1(FGF-1)由于氨基端缺乏信号肽序列,不能通过经典的内质网-高尔基体途径释放。
- With common molecular biology technique,we cloned SCF gene including signal peptide coding sequence coded signal peptide and constructed its expression plasmid. 采用常规的分子生物学技术,从小鼠骨髓细胞中克隆了含信号肽序列的SCF,并构建了表达载体。
- This article reviews signal peptide sequence about its structural feature,the common used signal peptide and the construction of vector with high secretory. 本文将从信号肽结构特征、常用的信号肽序列以及构建高分泌型载体等方面进行综述。
- Signal peptide was found on former 20 amino acids of both two coding regions of protein, one strong and one feeble transmembrane helices were also found. 2. 并在两种酶蛋白编码区的前20个氨基酸发现信号肽序列,通过跨膜区预测,均发现一个较强的跨膜信号和一个较弱的跨膜信号。
- The fusion DNA fragment of the lactococcal extracellular protease USP45 signal peptide, passenger protein and SBC were expressed in Lactococcus lactis and Lactobacillus paracasei. 融合乳酸菌胞外蛋白酶USP45讯息胜肽、乘载蛋白及表层蛋白质静默基因C端123个胺基酸,表现于副乾酪乳杆菌或乳酸链球菌。
- This dress is made of artificial fibers. 这件衣服是人造纤维的。
- Her artificial gaiety disguised an inner sadness. 她以矫揉造作的快乐掩饰著内心的悲痛。
- Obtaining of SPCEMA(signal peptide modified CEMA)SPCEMA(187bp) was amplified with two long complementary primers (P2 and P3) and two primers (Pl and P4) containing restriction enzyme recognition site. 以两条部分重叠长链引物P_2和P_3延伸产物为模板,P_1和P_4为正、反引物进行PCR扩增,获得了改造的抗菌肽基因SPCEMA(187bp)。