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- Study on the monoclonal antibody to chlorpyrifos. 抗毒死蜱单克隆抗体的研制。
- Methods Anti human bladder carcinoma cell line BIU 87 monoclonal antibody BDI 1 was labelled with technetium 99m by direct mercaptoethanol reducing method. 方法采用抗人膀胱癌单克隆抗体BDI-1通过2-巯基乙醇直接还原法标记99mTc(99mTc)制备了99mTc-BDI-1。
- After pretreatment of anti ICAM 1 and E selectin monoclonal antibody, serum LDH and LAT levels decreased significantly; KBR and ATP content of liver increased. 抗ICAM 1及E selectin单抗预处理可使急性胆管炎大鼠血浆LDH ,ALT水平明显降低 ,KBR及肝组织ATP含量升高。
- An alternative to the costly monoclonal antibody approach. 可替代昂贵的单克隆抗体分析方法。
- Objective: To express Fab fragments of the anti colonic cancer antibody and to compare the antigen binding characteristics between the expressing product and the authentic monoclonal antibody. 目的:在大肠杆菌中表达抗结肠癌抗体Fab片段,比较表达产物和原杂交瘤单克隆抗体的抗原结合特性。
- Results 98% purity was reached through 14F7 monoclonal antibody column, and 95% purity was gained after goat anti human Fab fragment column. But yield ratio of the two affinity columns was low, being 35% and 55%, respectively. 结果3种纯化方法中,14F7单克隆抗体柱纯化的Fab抗体的纯度达98%25左右,Fab抗体柱纯化的Fab抗体的纯度为95%25,但这两种亲和柱的目的蛋白收率都不高,分别为35%25、55%25。
- The results of detection of specific IgG4 in sera of 173 cerebral cysticercosis patients by dip test using gold labeled anti human IgG4 monoclonal antibody(McAb) were reported and compared with the case CT and MR scans. 应用金标抗人IgG4 McAb 浸测试验检测173 例脑囊虫病患者血清中特异IgG4,并与其脑CT、MR影像分型进行对比分析。
- Objective To develop monoclonal antibody Fab fragments using display technique. 目的 利用噬菌体抗体库技术制备抗甲状旁腺激素相关蛋白 (PTHrP)Fab抗体。
- Methods Immunohistochemistry using PCNA monoclonal antibody PC10 cvere studied. 方法用增殖细胞核抗原(PCNA)单克隆抗体PC10免疫组化研究。
- The neurites were also Identified by immunochemical staining using monoclonal antibody to neurofilament. 利用免疫化学染色,这些生长的神经轴突可被对抗神经细丝的单株抗体所染色。
- After anti human BPI polycolonal antibodies or monoclonal antibodies having no bactericidal activities were added, bactericidal rate of porcine BPI increased. But the antibody not against BPI had no such improving bactericidal activity. 抗人BPI多克隆抗体和单克隆抗体加入后 ,猪源BPI的杀菌活性不仅不降低 ,反而增加 ,而抗体本身并不具有杀菌性 ,非抗BPI抗体也无此效应 ;
- Methods The effects of anti CD4 human mouse chimeric and mouse derived monoclonal antibody on CD3, PHA, IL 2, proliferation induced by allogenic cells and the propagation of ADCC mediated by these antibodies were observed. 方法 观察CD4人 鼠嵌合抗体和鼠源性单抗对CD3、植物血凝素 (PHA)、白细胞介素 2 (IL 2 )及同种异体细胞诱导增殖的影响 ,以及其介导的抗体依赖性细胞介导的细胞毒性 (ADCC)作用。
- Objective: To study Bio-panning of hepatitis G virus(HGV) E2 with monoclonal antibody(mAb) from a nonapeptide library. 目的:筛选能识别庚型肝炎病毒(HGV)E2区的单克隆抗体和有竞争抑制活性的多肽。
- Campbell AM. Monoclonal antibody and immunosensor technology[M]. Elsevier Science Publisers BV, 1991, 189-203. 李成文.;现代免疫化学技术[M]
- Methods Immunoreactivity of monoclonal antibody JSB1 to P-glycoprotein were determined in brain tumors of children. 方法用单克隆抗体JSB1及免疫组化法检测P-糖蛋白。
- Immunohistochemical reagents: Mouse anti-rat BrdU monoclonal antibody was bought from Sigma Company. 免疫组织化学染涂色试剂:小鼠抗大鼠溴核苷脱氧嘧啶单克隆抗体购自Sigma公司;
- Objective: To obtain human monoclonal antibody Fab fragments(Fabs) against HBsAg from combinatorial libraries. 目的:制备人源性重组HBsAg抗体Fab片段。
- Objective:To clone Fab genes of anti-p185 monoclonal antibody 5E12 and express it in E. coli. 目的克隆抗p185单抗5E12的Fab段基因并在原核细胞进行表达。
- Fas antigen expression on BGC-823 cell surface was detected with Fas monoclonal antibody and then analyzed by FCM. 用Fas单克隆抗体检测BGC-823细胞表面Fas的表达;
- METHODS Assay of Ki 67 MIB 1 was done by a monoclonal antibody immunohistochemical method. 方法 应用Ki?67 MIB?1 免疫组织化学方法,检测其在脑膜瘤中的表达。