- Methods: HE,VG,Alcian blue stainings of wound tissues,computer image analysis.
- The deposition of mucin in the follicle was demonstrated by Alcian blue staining.
- Methods: Special stainings of toluidine blue, Alcian blue and electron microscopic technique were used in the study.
- The production of matrix was estimated based on hydroxyproline quantity and Alcian Blue stain method.
- Histologic examination showed that the dermis was thickened and the alcian blue staining was positive.
- The case is definitely diagnosed as REM according to clinical feature, results of histopathology and alcian blue staining.