- The purified OGT fusion protein displayed as a single band in SDS-polyacrylamide gel and had enzymatic activity, but the activity lost after it was eluted from the agarose beads.
其纯化的 OGT在电泳中显现单一条带并具有生物活性 ,但其活性在洗脱后丧失。
- A PET32a vector containing human OGT cDNA-S tag was expressed in BL21 E. Coli and the recombinant protein was purified with S-protein conjugated agarose beads.
携带人 OGTc DNA的 PET32 a质粒在大肠杆菌中表达出带有 1个 S- Tag的重组人 OGT融合蛋白 ,然后用与 S-蛋白质偶联的琼脂糖凝胶颗粒从细菌裂解液中亲和纯化 OGT融合蛋白。
- Ziye Liu, Jianbo Zhang, Xi Chen and Peng George Wang, "Combined biosynthetic pathway for de novo production of UDP- Galactose: Catalyzed with multiple enzymes immobilized on agarose beads.
- Methods The MT1 MMP hTAP protein complexes were isolated and purified using the IgG agarose beads and calmodulin sepharose beads affinity chromatography according to the molecular and biological features of TAP system.
方法 应用TAP系统分子生物学特性采用IgG和钙调蛋白亲和胶粒二次亲和层析法分离纯化MT1 MMP hTAP蛋白复合物。
- The hat was decorated with colored beads.
- He took a bead on the hare and then fired.