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- The adherent cells were gained by cultivated in vitro. 体外培养以获得贴壁细胞。
- Applicable to primary cells (HUVEC, primary Neurons, etc.) And other adherent cell lines. 用于原代细胞(人脐静脉内皮细胞,原代神经细胞等)和其他贴壁细胞系。
- The experiment revealed that in order to induce antibody in vitro, the adherent cells were necessary. 此实验还证明体外诱生抗体必须有粘附细胞参与。
- The suppressive activity of non-adherent cells (T cells) and adherent cells could be detected, too. 将去除了巨噬细胞的带瘤小鼠脾细胞进一步经尼龙毛分离,发现尼龙毛粘附细胞及非粘附细胞均产生一定的抑制作用。
- There are 8.825 + 2.958 adherent cells per 100 peripheral blood mononuclear cells. 每100个外周血单个核细胞(PBMC)可获得8.;825±2
- Culture of Primary Adherent Cells and a Continuously Growing Nonadherent Cell Line Cheryl D. 介绍细胞培养的基础知识。英文教材,PDF文件。
- The concentration ofcalcium was assessed by ACAS (adherent cell analysis and sortingcytorneter). 用粘附式细胞仪测定细胞内钙离子浓度;
- Cord blood adherent cells display fibroblast-like or spindle shape,and their reproductive cycle: G_0/G_1was 85.12%, the doubling time was 48 hours. 脐血贴壁细胞呈长梭形、针形或三角形;增殖周期G0/G1为85.;12%25;倍增时间为48h。
- After culture them for 14 days, collect adherent cells ,detect CD86,CD83, CD14, HLA-DR and other immune factors with flow cytometer. 14d后,收集贴壁DC细胞,分别用流式细胞技术检测CD83、CD86、CD14、HLA-DR等免疫表型。
- Methods The umbilical cord blood CD34 + cells were cultured in Dexter system in order to observe the biological behavior of adherent cells in vitro. 方法 分离人脐血CD34+ 细胞 ,采用Dexter法培养 ,对贴壁细胞进行观察和鉴定。
- Objective To study the growth characteristics of cord blood adherent cells and provide the hematopoietic microenvironment. 目的观察脐血贴壁细胞生长特性及提高体外培养脐血贴壁细胞成功率,为造血干细胞培养提供基质环境。
- The sample of single, live, adherent cell was studied by the confocal microscopy. 激光扫描共聚焦显微镜是以单个的、活性的、贴壁的细胞标本为主要的研究对象。
- The percentage of P210 positive cells was significantly lower in non adherent cells after 10 days of culture in Dexter system, comparing with that in non cultured BMMNC. BMMNC经培养后非贴壁细胞中P2 10阳性细胞比例明显减少 ,以含DC者最为显著 ,含rhIL 2者次之。
- Objective: To establish a method for isolation and purification of fetal membrane derived adherent cells (FMDACs) , and investigate their biological characteristics. 目的:建立体外分离纯化胎膜组织贴壁细胞(fetal membrane derived adherent cells, FMDACs)的方法,并且研究FMDACs的基本生物学特性。
- Immunocytochemistry and flow cytometry analysis showed that almost all of the adherent cells were vWF positive and around 90% were able to bind UEA I specifically. 免疫细胞化学和流式细胞术的结果显示 ,几乎所有贴壁细胞为vWF阳性 ,且约 90%25的细胞具有UEA I结合能力。
- The adherent cells and non-adherent cells were collected respectively,which were identified by light microscope,electron microscope, immunochemistry and flow cytometric analysis. 用光镜、电镜、细胞免疫化学、流式细胞仪法鉴定该两种细胞类型。
- GU XF, MA BY, WU Y, et al.Changes in the composition of adherent cells in long-term human bone marrow culture[J].ATCA Academiae medicinae shanghai, 1994, 21(5):369-372. [3]顾小锋;马伴吟;吴口;等.;人骨髓细胞体外长期培养基质细胞组成及其变化[J]
- Under appropriate conditions, the adherent cells from HUCB could form the feeder layer to support long-term hematopoiesis and provide the supporting stromal cells for the ex vivo expansion of HSPCs. 在适当的条件下;可以形成饲养层细胞;为造血干/祖细胞体外扩增提供支持基质细胞.
- METHOD VSMC were labelled with fluo 3 AM, a calcium sensitive fluorescent dye, and [Ca 2+ ] i was determined by the use of the adherent cells analysis and sorting apparatus(ACAS 570). 方法 用荧光染料Fluo 3 AM标记细胞 ,在粘附式细胞仪上测定细胞内游离钙的变化。
- The expression of neural and hematopoietic markers in placenta tissue and cultured human placenta derived adherent cells(hPDACs) and their correlation in embryonic development were investigated in this paper. 为探讨人胎盘组织在造血中的作用以及研究造血和神经标志物在胎盘中的表达和在胚胎发育中的相关性,用免疫组织化学法对人胎盘组织和体外培养的胎盘组织中的贴壁细胞进行染色,观察其造血因子和神经标志物的表达。