add serum contained nilestriol to rOB cultured in vitro, detect expression of Smad4 mRNA and protein in the supernatant fluid of rOB culture with the method of RT-PCR and Western blot.

• 以补肾健脾药物吸收后药物血清,加入体外培养的rOB中进行培养,分别以RTPCR法及Westernblot法检测rOB的Smad4mRNA和蛋白的表达。