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- Objective:To express ancrod,a snake venom thrombin-like enzyme,in methylotrophic yeast Pichia pastoris. 目的:利用毕赤酵母表达具有生物学活性的蛇毒类凝血酶安克洛。
- The methylotrophic yeast Pichia pastoris is now one of the important tools used in molecular biology for the generation of recombinant protein. 巴斯德毕赤酵母目前已经成为分子生物学研究中,表达外源蛋白的一种重要工具。
- Secondly, we selected yeast Pichia pastoris to be the gene-expressing host and the secreting plasmid pPIC9 was as vector to construct recombined plasmid pPIC-Atmp. 研究中,我们选用巴斯德毕赤酵母作为基因表达系统,分泌型质粒pPIC9作为载体,构建了基因工程菌。
- Qiu D R,Zhu J P,Wang L,et al.Expression of recombinant human p53 in methylotrophic yeast pichia pastoris[J].Journal of Biotechnology,1999,15(4):477-481. [8]邱荣德;朱建蓓;王垒;等.;人p53蛋白在巴氏德毕赤酵母中的表达[J]
- The ScFvA1-Etag and the glucose-linker-ScFvA1 (GLS) fusion gene were cloned into the vector pPIC9K and expressed in methylotrophic yeast Pichia pastoris GS115, respectively, under the control of the AOX1 promoter. 将ScFvA1-Etag基因,GLS融合基因重组进大肠杆菌-酵母穿梭质粒pPIC9K, 分别构建含有ScFvA1-Etag基因,GLS融合基因的表达载体pPIC9K-ScFvA1-Etag,pPIC9K-GLS。
- Because it is hard to industrify the production of the targeting-medicant by this method ,we decide to research the expression of apoAI gene in methytrophic yeast Pichia pastoris system . 因此,我们决定运用毕赤氏酵母表达系统进行ApoAI的表达研究,将获得的rApoAI作为一个新的载体,为制备新型的靶向药物创造条件。
- Methylotrophic yeast Pichia pastoris 毕赤甲醇酵母
- Keywords Yeast Pichia pastoris;Chitotriosidase;Fungus;Chitin; 关键词毕赤酵母;壳三糖酶;真菌;几丁质;
- Advances on genetic engineered antibody expression in the yeast Pichia pastoris 基因工程抗体在巴斯德毕赤酵母中表达的研究进展
- Keywords HBsAg;GM-CSF;Fusion protein;Yeast Pichia pastoris;Immunogenicity; 融合蛋白;毕赤酵母;免疫原性;
- Expression of Serine Mutant of Microplasminogen at Active Site in Methylotrophic Yeast Pichia pastoris 人微小纤溶酶原活性中心丝氨酸突变体基因在毕赤酵母中的表达
- The Expression of Humanized Fab Fragment of the Anti-HBsAg Antibody in Methylotropic Yeast Pichia pastoris 人源性抗HBsAg抗体Fab段在酵母中的表达
- Expression, purification and identification of Alpha subunit of rabbit SO cell BK channel in methylotrophic yeast Pichia pastoris 兔Oddi氏括约肌细胞BK通道Alpha亚基在Pichia酵母中的高效表达、纯化和鉴定
- Yeast Pichia pastoris 毕赤酵母
- In Basal Salts Medium, the yeast grows up to A600=76 inoculated 10% seed pichia pastoris with A600=8-12 in 24h. 在无机盐培养基中,按10%25接种A600=8-12的种菌后,24小时酵母菌增殖至A600=76;
- Aim To construct the Pichia pastoris expression system of RS gene. 目的建立白藜芦醇合酶基因的毕赤酵母表达系统。
- With high yields of rHSA and relative simple extraction technology,the yeast expression system,especially Pichia pastoris system,is the most prospect system in industrialization. 用酵母表达系统,尤其是毕赤酵母,表达的重组白蛋白产量高且提取工艺简单,是其产业化最具有前途的表达系统。
- This review focused on progress of Pichia pastoris yeast expression system, the technique of separation and purification of the recombinant proteins recently. 简单介绍了目前较为流行的毕赤酵母表达体系,着重概述了重组蛋白分离纯化技术方法的应用情况。
- Intracellular expression of cGHcGH eDNA was cloned into the yeast Pichia paslOri. 1 cGH在毕赤酵母细胞内的表达
- AIM: To express human anti-keratin Fab in Pichia pastoris secretively and optimize the expression condition. 目的:用巴氏毕赤酵母表达抗角蛋白抗体Fab段并优化表达条件。