With the concentration of l~3mg/L 2,4-D and after five subculture periods, the calli were vivid light yellow granules and grew fast, which was ideal to establish suspension culture.

• 2,4-D浓度介于1mg/L和3mg/L之间，经过5次调控继代的愈伤组织，形成的愈伤组织由色泽淡黄而鲜艳的小颗粒组成，生长速度快，适宜建立胚性悬浮细胞系。