With an intra control, Wx-Blb allele can be detected using MAG266 and MAG267. The primers of marker MAG269 amplified a 1400bp band in the wild type of the Wx-Dl locus, and a800bpbandinthemutantallele.

• 结合内对照，利用MAG266和MAG267标记能够特异地识别Wx-B1a等位基因。