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- Method:Using Western blot method to measure HSP of hypothalamus in febrile and hypothermal rats. 方法:取高、低体温大鼠下丘脑组织,应用蛋白印迹法测定热休克蛋白含量。
- M ethod:Using Western blot method to measure HSP of hypothalamus in febr ile and hypothermal rats. 方法:取高、低体温大鼠下丘脑组织,应用蛋白印迹法测定热休克蛋白含量。
- The expression state of p53, Bcl-2 and Bax of rat hepatocytes BRL-3A as exposed to microcystin LR was studied with western blot method. 用蛋白印迹法研究了微囊藻毒素LR(MCLR)暴露时大鼠BRL-3A细胞p53、Bcl-2和Bax的表达情况.
- Analyses of the changes of Calbindin-D28k(CB) expression and Glial Fibrillaiy Acidic Protein(GFAP) expression in rats hippocampus using method immunohistochemistry and western blotting method. 应用免疫组织细胞化学方法和western印迹技术分析大鼠海马结构的神经元内维生素D依赖性钙结合蛋白D28k(Calbindin-D28k,CB)和神经胶质细胞内胶质纤维酸性蛋白(Glial Fibrillary Acidic Protein, GFAP)表达的变化。
- The relationship between certain protein expressions(MLH1,ERCC1,PARP,RAD51,XRCC4) and CQ increased radiosensitivity in radioresistant MDA-MB 231 cells was examined by Western blot method. 用W estern b lot印迹杂交法检验与DNA修复有关的5种基因(MLH1,ERCC1,PARP,RAD51,XRCC4)蛋白表达与CQ增加辐射抗拒细胞辐射敏感性的关系;
- The human colon cancer LoVo cells were transfected by Lipofectamine 2000 with plasmid vector, and then selected for 4 weeks by G418. The expression of cerb-B1 mRNA was assessed by real time PCR and the protein of EGFR was assessed by Western blot method. 应用脂质体Lipofectamine2000转染人结肠癌LoVo细胞,转染成功后以G418筛选4周,实时荧光定量RT-PCR(RealTimeRT-PCR)检测cerb-B1基因的mRNA表达,Western-blot检测EGFR蛋白的表达。
- The expression of CLC-3 mRNA and CLC-3 protein were tested by reverse transcription-polymerase chain reaction (RT-PCR) and western blot methods. 应用逆转录聚合酶链反应(RT-PCR)、免疫印迹(Western blot)方法检测CLC-3 mRNA和蛋白的表达。
- METHODS: We used immunohistochemical ABC and Western blot method to detect the expression of p38 MAPK in lung tissues and airway epithelial cells in acute lung injury rat model and observed the influence of N acetylcysteine (NAC) on the expression. 方法 :采用免疫组织化学ABC法和Western blot技术 ;检测磷酸化的 p38MAPK在大鼠急性肺损伤模型气道和肺组织的表达 ;并同时观察N 乙酰半胱氨酸 (NAC)对其表达的影响 .
- Methods By using Northern blot and Western blot methods,the expression of gene and protein of VEGF-A,-C in all 6 tested cell lines (ASPC-1,CAPAN-1,MIA-PaCa-2,PANC-1,COLO-357 and T3M4) was detected respectively. 方法 用Northern杂交和Western杂交法分别分析人胰腺癌细胞株(ASPC 1、CAPAN 1、MIA PaCa 2、PANC 1、COLO 3 5 7和T3M 4)中VEGF A、C基因和蛋白的表达;
- Methods Using RT-PCR and Western Blot methods,the changes of AMPA receptor GluR2 subunit mRNA and protein expressions were observed in cultured hippocampal neurons of rats induced by glutamate. 方法用RT-PCR和Western Blot方法观察谷氨酸诱导培养大鼠海马神经元AMPA受体Glu R2亚单位mRNA和蛋白的表达变化。
- Keywords Cataract;Epithelial cells;Lens;Vimentin;Immunohistochemistry;Western blot method;protein; 白内障;上皮细胞;晶体;波形纤维蛋白;免疫组织化学;印迹法;蛋白质;
- The expression of bFGF protein in LECs of normal person and cataract patients cultured in vitro at different passages were detectd by immunohistochemical staining and Western blots methods. 利用免疫组织化学法和免疫印迹法,检测bFGF在体外培养的正常人及白内障患者LECs不同阶段的蛋白水平的表达。
- This CTB gene product was also verified by Western blot analysis. Western blotting结果确认了该条带为CTB基因的产物。
- Western blot showed 60 kDa, which may be posttranslated product. Western blot 结果显示,该基因的真核表达产物分子量约为60 kD,可能是翻译后修饰的结果。
- Method The distribution and amount of CX43 was investigated in different brain area and different period with immunohistochemistry and Western blot. 方法 利用免疫组织化学方法、Westernblot方法测定KA致痫后大鼠不同脑区、不同时程的CX43的分布及表达。
- Method:Expression of iNOS protein in injured spinal cords was detected by Western blot after 1,2,3,5,7 days after injury (n=3) with Allen's weight dropping model. 方法 :Allen's模型致伤后 ,分别于损伤后 1、2、3、5、7d取伤段脊髓 ,作蛋白质印迹分析。
- Expression of the M 2-1 gene was examined by RT PCR and Western blot. 重组真核质粒PXJ 4 1/M2 -1转染肺腺癌PAa细胞 ,应用RT PCR、Westernblot方法检测表达。
- In this study the authors detected both nm23 H 1 and nm23 H 2 mRNA levels in 52 tissues samples of patients with carcinoma of buccal mucosa (CBM) by Northern blotting method. 通过Northern斑点杂交技术研究52例颊癌及相关组织中肿瘤转移抑制基因nm23-H1和nm23-H2mRNA的表达。
- The results of western blot analysis were in accordance with those of 2-DE. 对部分差异蛋白采用Western-blot方法在蛋白水平的验证结果与2-DE结果基本相符。
- Western blot was used to analyze a panel of HSP90 client proteins expression. Western blot分析药物对细胞通路信号蛋白及细胞周期相关蛋白的表达;
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- 深远海浮式风电平台 - deep-sea floating wind power platform
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- 京雄高速公路 - Beijing-Xiongan expressway
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- 农业及相关产业增加值 - the added value of agriculture and related industries