Western blotting test and immunofluorescence showed that E2 gene could be expressed in COS7 cells. pcDNA_E2 induced specific antibody against EEEV in immunized mice.

• 免疫印迹法、免疫荧光法检测表明E2基因在COS7细胞中获得瞬时表达 ,pcDNA_E2基因免疫小鼠可产生抗东部马脑炎病毒的特异抗体。