Western blot analysis was used to detect pl85 HER-2, HER-2 ECD and phospho-HER-2. Two-site ELISA assay was used for the detection of HER-2 ECD.

• 双抗夹心ELISA法检测细胞培养上清中HER-2 ECD水平,Western blot检测细胞HER-2蛋白水平。