We used mouse germinal vesicle stage oocyte cultured with special concentration progesterone, or/and antisense c-myb ODN, or/and dbcAMP, or/and Verapamil, or/and heparin for 24h, and observed oocyte maturation and analysed the relationship between them.

• 在适宜浓度孕酮存在下,将小鼠GV 期卵母细胞或/和反义c-myb ODN、或/和二丁酰环腺苷酸(dbcAMP)、或/和维拉帕米(Verapamil)、或/和肝素钠(Heparin)共同孵育24h,观察卵母细胞体外成熟的情况并分析它们之间的相互关系。