We have developed a method of in situ hybridization to study the distribution of Coxsackie virus B3(CVB3) genome in the frozen organ sections of mice infected with CVB3 at different intervals in a period of 12 days postinfection.

• 用柯萨奇B3病毒（CoxsackievvirusB3，CVB3）cDNA探针原位杂交法检测感染后12d内小鼠各脏器内病毒核酸的分布。