V1/V2= (r1/r2) 2 where V1and V2 is the diameter for analytical and preparative column respectively, r1 and r2 is the flow rate for analytical and preparative HPLC respectively.

 
  • 小中大色谱柱直径变大的化,流速也要跟着放大,保持线速度不变,分离效果变化很小。
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